山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (5): 43-46.doi: 10.6040/j.issn.1673-3770.0.2015.290

• 论著 • 上一篇    下一篇

变应性鼻炎患者外周血EOS-CSF、IL-5水平及EOS数目对局部皮质类固醇激素治疗效果的评价

张辉1, 郑成彩2, 冯慧伟1, 郭成浩3   

  1. 1. 山东大学第二医院耳鼻咽喉头颈外科, 山东 济南 250033;
    2. 日照市东港区人民医院耳鼻咽喉科, 山东 日照 276800;
    3. 山东大学医学院, 山东 济南 250012
  • 收稿日期:2015-07-13 修回日期:2015-09-21 出版日期:2015-10-16 发布日期:2015-10-16
  • 通讯作者: 郭成浩,E-mail:chenghaoguo@sdu.edu.cn E-mail:chenghaoguo@sdu.edu.cn
  • 作者简介:张辉,E-mail:lucy_zhang@sdu.edu.cn
  • 基金资助:
    山东省科技攻关项目(2009gg10002025)

Valuation of the therapeutic effect of the nasal steroid hormone spray to detect EOS-CSF, IL-5 levels, EOS number of peripheral blood in allergic rhinitis patients

ZHANG Hui1, ZHENG Chengcai2, FENG Huiwei1, GUO Chenghao3   

  1. 1. Department of Otolaryngology & Head and Neck Surgery, The Second Hospital of Shandong Uuniversity, Jinan 250033, Shandong, China;
    2. Department of Otolaryngology, The Donggang Hospital of Rizhao City, Rizhao 276800, Shandong, China;
    3. The Medical School of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-07-13 Revised:2015-09-21 Online:2015-10-16 Published:2015-10-16

摘要: 目的 检测持续性中重度变应性鼻炎(AR)患者局部应用皮质类固醇激素治疗前后外周血中嗜酸粒细胞集落形成因子(EOS-CSF)、白介素5(IL-5)的水平,判断AR症状缓解以及临床药物治疗效果评价的方便快速的实验室指标。方法 研究分AR组、AR治疗4周组、AR治疗12周组及正常对照组。采用ELAISA方法检测外周血EOS-CSF、IL-5水平,常规计数外周血嗜酸粒细胞(EOS)。应用视觉模拟量表(VAS)评价患者的鼻部症状。结果 AR组血中EOS-CSF和IL-5水平及外周血中EOS数目明显高于正常对照组。AR治疗4、12周组与AR相比,IL-5和EOS-CSF水平及外周血中EOS数目都明显降低。患者血清IL-5水平与EOS计数呈正相关(r=0.63, P<0.05);血清EOS-CSF水平与EOS计数呈正相关(r=0.68, P<0.05)。结论 经鼻用类固醇激素治疗后,检测外周血EOS-CSF和IL-5及查外周血EOS数目可用以监测变应性鼻炎治疗效果并对调整用药提供指导。

关键词: 鼻炎, 嗜酸粒细胞集落形成因子, 嗜酸粒细胞, 变应性, 白介素5

Abstract: Objective To detect the levels of eosinophil colony stimulating factor (EOS-CSF), interleukin-5(IL-5) and eosinophil(EOS) numbers in persistent moderately and severe allergic rhinitis patients'peripheral blood and to investigate the relationship between the levels of EOS-CSF and IL-5 and the clinic symptom of AR patients after using the Mometasone Furoate Aqueous Nasal Spray. Methods The levels of EOS-CSF and IL-5 in peripheral blood serum were detected by enzyme linked immunosorbent assay (ELISA). The number of EOS of fresh blood smear were observed by stained by Wright Giemsa method. The patients' nasal symptoms were evaluated by visual analogue scale. Results The levels of IL-5 and EOS-CSF and the EOS number in the AR group were significantly higher than in the normal control group.There was significant reduction of IL-5 and EOS-CSF in the patients after therapy. The data showed that the IL-5 level and EOS-CSF were positively correlated with EOS number. Conclusion Detection EOS-CSF, IL-5 and EOS number of peripheral blood in AR patients after treatment with nasal steroid hormone can be used to monitor the efficacy of treatment and provide guidance to adjust medication.

Key words: Allergic rhinitis, Interleukin 5, Eosinophil, Eosinophil colony stimulating factor

中图分类号: 

  • R765.4
[1] Brozek J L, Bousquet J, Baena-Cagnani C E, et al. Allergic rhinitis and its impact on asthma (ARIA) guidelines: 2010 Revision[J]. J Allergy Clin Immunol, 2010, 126(2):466-476.
[2] Seidman M D, Gurgel R K, Lin S Y, et al. Clinical practice guideline: allergic rhinitis[J]. Otolaryngol Head Neck Surg, 2015, 152(1 Suppl):S1-43.
[3] ZHAO J, BAI J, SHEN K, et al. Self-reported prevalence of childhood allergic diseases in three cities of China:a multicenter study[J]. BMC Public Health, 2010, 10(3):551-551.
[4] 欧阳昱晖,张罗,韩德民. 慢性嗜酸细胞性鼻及鼻窦炎[J].中国耳鼻咽喉头颈外科, 2010,17 (1):53-55. OUYANG Yuhui, ZHANG Luo, HAN Demin. Eosinophilic chronic rhinosinusitis[J]. Chin Arch Otolaryngol Head Neck Surgery, 2010, 17(1):53-55.
[5] 张辉,张大良,赵敬杰,等. 变应性鼻炎患者外周血嗜酸粒细胞一骨髓干细胞通路指标检测在激素治疗评价中的意义[J].中华耳鼻咽喉头颈外科杂志,2008,43(7):490-493. ZHANG Hui, ZHANG Daliang, ZHAO Jingjie, et al. Significance of the examination of path between eosinophils in peripheral blood and marrow stem cells for evaluating the effect of hormone therapy in patients with allergic rhinitis[J]. Chin J Otolaryngol Head Neck Surgery, 2008, 43(7):490-493.
[6] 张辉,张大良,郭成浩,等.变应性鼻炎患者外周血CD34和白介素5及嗜酸粒细胞的检测[J]. 中华耳鼻咽喉头颈外科杂志,2005,40(7):541-542. ZHANG Hui, ZHANG Daliang, GUO Chenghao, et al. detection of CD34, interleukin 5 and eosinophils in patients with allergic rhinitis in the peripheral blood[J]. Chin J Otolaryngol Head Neck Surgery, 2005, 40(7):541-542.
[7] 中华耳鼻咽喉科杂志编委会,中华医学会耳鼻咽喉科学分会. 变应性鼻炎的诊断和治疗指南[J].中华耳鼻咽喉头颈外科杂志,2009, 44(12):977-978. Editorial Board of Chinese Journal of Otorhinolaryngology, Chinese Medical Association of Otorhinolaryngology. Guidelines for the diagnosis and treatment of allergic rhinitis[J]. Chin J Otolaryngol Head Neck Surgery, 2009, 44(12):977-978.
[8] 施典羽,杨凯,敬光怀,等.布地奈德鼻喷剂对变应性鼻炎患者血中IL-5和IL-12的影响[J]. 安徽医学,2011,32(5):641-642. SHI Dianyu, YANG Kai, JING Guanghuai, et al. The effect of budesonide nasal spray on the IL-5 and IL-12 in the patients with allergic rhinitis[J]. Anhui Med, 2011, 32(5):641-642.
[9] Krouwels F H, Hol B E, Lutter R, et al. Histamine affects interleukin-4, interleukin-5, and interferon-gamma production by human T cell clones from the airways and blood[J]. Am J Resp Cell Mole Miol, 1998, 18(5):721.
[10] Sanderson C J. Interleukin-5, eosinophils, and disease[J]. Blood, 1992, 79(12):3101-3109.
[11] 燕志强,高盛宏,张俊,等.糖皮质激素对变应性鼻炎大鼠血清IL5、IL12、IL18含量变化的影响[J]. 解放军医学杂志,2009,34(10):1218-1220. YAN Zhiqiang, GAO Shenghong, ZHANG Jun, et al. Effect of glucocorticoid on the contents of IL-5, IL-12, IL-18 in allergic rhinitis rats[J]. Med J Chin PLA, 2009, 34(10):1218-1220.
[12] 韩德民.加强变应性鼻炎研究促进临床规范治疗[J].中华耳鼻咽喉头颈外科杂志,2006,41(7):481-482. HAN Demin. Strengthen the research of allergic rhinitis and promote the clinical standard treatment[J]. Chin J Otolaryngol Head Neck Surgery, 2006, 41(7):481-482.
[1] 王坛,武珂,李连庆,宫丽丽. 皮下免疫治疗注射后出现全身不良反应的伴发因素及处理[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 71-74.
[2] 卢汉桂,林歆胜,姚丹勉,魏永新,李创伟. 变应性鼻炎大鼠IL-35的表达及对辅助性T细胞免疫调节的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 66-70.
[3] 浦洪波,杜晓东. 无锡地区2 000例变应性鼻炎变应原检测结果分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 105-107.
[4] 陈鸣,俞雪飞. 浅谈伴有变应性鼻炎的慢性鼻窦炎的治疗[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 18-22.
[5] 吴湘萍. 患者管理方式对变应性鼻炎舌下含服粉尘螨滴剂疗效的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 68-72.
[6] 李松,王宗贵,杨景朴,张竹萍. 鼻内镜下翼管神经切断术进展[J]. 山东大学耳鼻喉眼学报, 2018, 32(1): 72-76.
[7] 郅莉莉,宋道亮. 嗜酸性粒细胞及IL5在上颌窦后鼻孔息肉与鼻息肉中表达的差异[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 43-46.
[8] 万文锦,王文,程雷. 尘螨变应性鼻炎皮下免疫治疗与舌下免疫治疗的荟萃分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 103-108.
[9] 朱新华. 变应性鼻炎冲击免疫治疗的临床应用[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 13-17.
[10] 史丽,赵莉,张红萍. 变应性鼻炎的长期抗炎治疗[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 9-12.
[11] 刘静. 基于整体观念变应性鼻炎的中医辨证论治[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 28-30.
[12] 接英,卢红双. 变应性结膜炎的诊疗策略[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 31-36.
[13] 程雷,钱俊俊,田慧琴. 变应性鼻炎研究的若干进展[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 1-3.
[14] 许昱,陶泽璋. 重视变应性鼻炎药物治疗的规范化和个体化[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 4-8.
[15] 刘英,叶小静,赵春丽,季青,黄谦. 季节性变应性鼻炎预防性治疗观察[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 37-40.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!