山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (4): 26-29.doi: 10.6040/j.issn.1673-3770.0.2016.032

• 变应性鼻炎 • 上一篇    下一篇

变应性鼻炎部分相关单体型模块基因单核苷酸多态性研究

滕伟强1,陈晓平2,张燚2,周恩晖3,姚文昊3,李力1   

  1. 1.上海长征医院耳鼻咽喉头颈外科, 上海 200003;
    2.上海市浦东新区公利医院耳鼻喉科, 上海 200135;
    3. 宁夏医科大学, 宁夏 银川 750004
  • 收稿日期:2016-01-24 出版日期:2016-08-16 发布日期:2016-08-16
  • 通讯作者: 陈晓平. E-mail:chen_xp2000@163.com E-mail:369313602@qq.co
  • 作者简介:滕伟强. E-mail:369313602@qq.co

Study on single nucleotide polymorphisms of partial allergic rhinitis-associated haplotype module genes.

TENG Weiqiang1, CHEN Xiaoping2, ZHANG Yan2, ZHOU Enhui3, YAO Wenhao3, LI Li   

  1. 1. Department of Otolaryngology &Head and Neck Surgery, Shanghai Changzheng Hospital, Shanghai 200003, China;2. Department of Otolaryngology &Head and Neck Surgery, Gongli Hospital of Pudong Area, Shanghai 200135, China;3. Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Received:2016-01-24 Online:2016-08-16 Published:2016-08-16

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摘要： 目的 检测患者染色体上有无变应性鼻炎相关基因单体型模块SDAD1、CXCL10、CXCL11、EoBL的存在,有无单核苷酸多态性(SNP)的存在及遗传因素对这些SNP有无影响。 方法 提取有遗传背景组和散发组的变应性鼻炎患者的血液样本DNA,通过PCR引物设计、合成、扩增后进行基因测序。 结果 实验在2组中一共检测出46个位点,其中发生突变的位点有34个,总的突变率为78.3%。变应性鼻炎的基因突变在有遗传背景组(66.67%)和散发组(78.57%)之间差异无统计学意义(P=0.58)。 结论 变应性鼻炎患者染色体上单体型模块SDAD1, CXCL9, CSCL10及CXCL11基因连续表达,变应性鼻炎相关基因SDAD1、CXCL10、CXCL11、EoBL上有SNP的广泛存在,但遗传因素对这些突变无明显的影响。

关键词: 变应性鼻炎, 单体型模块基因, 单核苷酸多态性

Abstract: Objective To detect whether there exist allergic rhinitis-associated hapoltype module genes(SDAD1, CXCL10, CXCL11 and EoBL)and single nucleotide polymorphisms(SNP)on chromosome as well as whether these genes affect on SNP. Methods The blood sample DNA of patients with allergic rhinitis in hereditary background group and distribution group was extracted, and gene sequencing was conducted following polymerase chain reaction(PCR)primer design, synthesis and amplification. Results A total of 46 sites were detected in both groups, of which 34 had mutations, with total mutation rate being 78.3%. And there was significant difference in the gene mutation of allergic rhinitis between hereditary background group(66.67%)and distribution group(78.57%)(P=0.58). Conclusion SDAD1, CXCL9, CSCL10 and CXCL11 genes are expressed successively on chromosome haplotype modules in patients with allergic rhinitis, and SNP is extensively distributed on allergic rhinitis-associated genes, including SDAD1, CXCL10, CXCL11 and EoBL, but hereditary factors have insignificant influence on these mutations.

Key words: Haplotype module genes, Single nucleotide polymorphisms, Allergic rhinitis

中图分类号: 

  • R765.21
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