ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (6): 74-78.doi: 10.6040/j.issn.1673-3770.0.2017.374
张颖,陈红梅,吴欣怡
ZHANG Ying, CHEN Hongmei, WU Xinyi.
摘要： 目的 研究糖尿病大鼠角膜上皮创伤愈合过程中胰岛素样生长因子-1(insulin-like growth factor-1, IGF-1)及胰岛素样生长因子-1受体(insulin-like growth factor-1 receptor, IGF-1R)的表达,从分子水平探讨糖尿病角膜上皮创伤愈合延迟的可能机制。 方法 链脲霉素诱导糖尿病大鼠模型,造模后每周同一时间点监测体质量和空腹血糖变化。制作大鼠角膜上皮创伤模型,于创伤后不同时间点,评价角膜上皮愈合速率;实时定量PCR及免疫荧光检测角膜上皮中IGF-1、IGF-1R的表达。 结果 糖尿病组体质量低于正常组,空腹血糖浓度高于正常组。与正常组相比,糖尿病组角膜上皮愈合速率慢。实时定量PCR及免疫荧光检测显示,新愈合的糖尿病组大鼠角膜上皮中IGF-1、IGF-1R的mRNA、蛋白表达低于正常组。 结论 糖尿病大鼠角膜上皮创伤愈合延迟,IGF-1、IGF-1R表达下调可能是导致愈合延迟的原因之一。
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