MicroRNA-24对人喉鳞癌细胞生物学行为的影响

doi:10.6040/j.issn.1673-3770.0.2018.021

摘要/Abstract

摘要： 目的探讨MicroRNA-24(miR-24)对喉鳞状细胞癌(LSCC)Hep-2、AMC-HN-8细胞增殖、侵袭及凋亡等生物学行为的影响。方法应用Lipofectamine^TM 2000转染上调Hep-2、AMC-HN-8细胞中miR-24的表达,通过荧光定量PCR验证转染效率;然后分别采用MTT法、克隆形成实验、细胞划痕实验、Transwell侵袭实验流式细胞分析技术分析外源上调miR-24后Hep-2、AMC-HN-8细胞增殖、迁移、侵袭及凋亡的影响。结果 miR-24质粒稳定转染Hep-2、AMC-HN-8细胞后miR-24表达明显上调。与转染miR-NC组和空白对照组相比,转染miR-24能明显降低Hep-2细胞的增殖、迁移和侵袭能力;同时,转染miR-24能明显增加Hep-2、AMC-HN-8细胞的凋亡能力。结论 miR-24异常表达与LSCC Hep-2、AMC-HN-8细胞的生物学行为密切相关,可能发挥抑癌作用。

关键词: 喉鳞癌, MicroRNA-24, 增殖, 迁移, 侵袭, 凋亡

Abstract: Objective The effect of miR-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells was examined. Methods After constructing Hep-2 and AMC-HN-8 cells to overexpress miR-24 by cell transfection, the proliferation of Hep-2 and AMC-HN-8 cells was detected by MTT assay and a cloning assay. A scratch test and Transwell assay were conducted to detect cell migration and invasion abilities, and the effect on apoptosis was analyzed by using flow cytometry analysis. Results After cell transfection, qRT-PCR confirmed upregulation of miR-24 in Hep-2/miR-24 and AMC-HN-8/miR-24 cells. MTT and colony formation assays showed that upregulation of miR-24 significantly reduced the proliferation of LSCC cells. Additionally, the capacity for colony formation in Hep-2/miR-24 and AMC-HN-8/miR-24 cells was significantly reduced compared to that of control cells. The scratch test and Transwell assay showed that the migration and invasion ability of miR-24 were significantly reduced. Flow cytometry showed that overexpression of miR-24 significantly enhanced apoptosis in LSCC cells. These results suggest that miR-24 inhibits the proliferation, invasion, and metastasis and increases the apoptosis of LSCC cells. Conclusion Abnormal expression of miR-24 is closely related to properties of LSCC cells. miR-24 inhibits the proliferation and migration and enhances the apoptosis of LSCC cells.

Key words: Laryngeal squamous carcinoma, MicroRNA-24, Inhibition, Proliferation, Invasion, Apoptosis

中图分类号:

R739.65

许莉,王志颐,陈伟,薛飞,张勇,程友,季俊峰,崔鹏程,江满杰. MicroRNA-24对人喉鳞癌细胞生物学行为的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 30-37.

XU Li, WANG Zhiyi, CHEN Wei, XUE Fei, ZHANG Yong, CHENG You, JI Junfeng, CUI Pengcheng, JIANG Manjie. Effect of MicroRNA-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(6): 30-37.

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