miR-204通过内质网应激抑制视网膜母细胞瘤生长作用及机制的研究

doi:10.6040/j.issn.1673-3770.0.2018.132

摘要/Abstract

摘要： 目的探讨miR-204在视网膜母细胞瘤中的表达、对其生长的作用及其分子机制。方法通过RT-PCR检测miR204 在视网膜母细胞瘤(Y79)及正常视网膜细胞(ARPE-19)中的表达,并用miR-204 模拟物及抑制物转染Y79 细胞,CCK-8 及流式细胞仪检测Y79 细胞的生存率及凋亡情况, Western blot 法检测GRP78蛋白的表达。结果 miR-204在视网膜母细胞瘤Y79细胞中呈现低表达,可显著抑制视网膜母细胞瘤的增殖,促进其凋亡,可上调 GRP78蛋白的表达,激活内质网应激途径。结论 miR-204在视网膜母细胞瘤细胞中表达下调,miR-204可能通过激活内质网应激介导的调亡途径诱导视网膜母细胞瘤细胞发生凋亡。

关键词: miR-204, 视网膜母细胞瘤, 内质网应激

Abstract: Objective To evaluate miR-204 expression in human retinoblastoma and to investigate the effect of latter's growth and molecular mechanism. Methods RT-PCR was performed to detect the expression of miR-204 in retinoblastoma(Y79)and the normal retinal(ARPE-19)cell lines. The mimic(s)and inhibitor(s)of miR-204 were transiently transfected into Y79 cells. CCK-8 and flow cytometry aided the detection of the survival and apoptosis of Y79 cells. The expression level of GRP78 protein was determined by Western blot. Results The expression of miR-204 in Y79 cells was significantly lower than that in ARPE-19. It was observed that the over-expressed miR-204 in Y79 cells could significantly inhibit the proliferation of retinoblastoma. Moreover, it promoted apoptosis, increased GRP78 protein's expression, and could activate the endoplasmic reticulum stress pathway. Conclusion miR-204 expression in retinoblastoma cells was significantly lower than that in the normal retinal cells. In addition, miR-204 might induce retinoblastoma cell(s)apoptosis by activating the endoplasmic reticulum stress pathway.

Key words: miR-204, Retinoblastoma, Endoplasmic reticulum stress

中图分类号:

R774.1

苏杰,马春梅,赵丽莉,刘岩,邵宏超. miR-204通过内质网应激抑制视网膜母细胞瘤生长作用及机制的研究[J]. 山东大学耳鼻喉眼学报, 2019, 33(4): 105-108.

SU Jie, MA Chunmei, ZHAO Lili, LIU Yan, SHAO Hongchao. Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 105-108.

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