ISSN 1673-3770 CN 37-1437/R
山东大学耳鼻喉眼学报 ›› 2019, Vol. 33 ›› Issue (5): 40-43.doi: 10.6040/j.issn.1673-3770.0.2019.063
姚青秀,王会,李壮壮,于栋祯(
),殷善开
Qingxiu YAO,Hui WANG,Zhuangzhuang LI,Dongzhen YU(),Shankai YIN
摘要： 筛选耳蜗毛细胞中与肌球蛋白VI相互作用的蛋白。 提取SD大鼠耳蜗蛋白，通过联合免疫共沉淀和质谱分析筛选与肌球蛋白VI相互作用的蛋白，挑选1种蛋白通过基底膜铺片和冰冻切片免疫荧光进一步验证。 免疫共沉淀及质谱分析筛选出与肌球蛋白VI相互作用的20余种蛋白质，免疫荧光显示血影蛋白αⅡ主要表达于表皮板及两侧胞膜，肌球蛋白VI主要表达于细胞体及表皮板，两种蛋白在表皮板均高表达。 本研究共发现20余种与肌球蛋白Ⅵ相互作用的蛋白质，提示肌球蛋白Ⅵ可能与多种蛋白相互作用维持静纤毛稳定。
中图分类号:
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