山东大学耳鼻喉眼学报 ›› 2019, Vol. 33 ›› Issue (5): 96-100.doi: 10.6040/j.issn.1673-3770.0.2019.468
Lu LUO,En ZHOU,Si OUYANG,Yi CHEN,Xuping XIAO,Jihua WANG()
摘要: 探讨42例喉癌患者血清microRNAlet-7a水平的变化及其对喉癌的诊断价值。 qRT-PCR检测42例喉癌患者(手术前后)及42例健康体检者血清miRNA let-7a的表达,分析血清let-7a水平与喉癌的临床病理因素的关系,用受试者工作曲线(ROC)分析其对喉癌的诊断效能。 血清let-7a在实验组比正常对照组相对表达下降分别为:0.38±0.0831、0.933±0.0723,差异有统计学意义;喉癌患者术前比术后1周血清let-7a的相对表达下降为:0.38±0.0831、0.488±0.1468,差异有统计学意义。血清let-7a的相对表达量与淋巴结转移有关联(P<0.05);与年龄、性别、饮酒、吸烟、浸润深度、肿瘤分型、TNM分期、分化程度无关联(P >0.05)。受试者工作曲线(ROC)显示以0.765为血清let-7a相对表达量的最佳临界值诊断喉癌,其灵敏度为76.5%,特异度为76.1%,AUC为0.850,95%置信区间为0.776~0.932。 喉癌患者血清miRNAlet-7a水平较健康者出现下调,let-7a有望成为诊断喉癌的新的分子标志物。血清let-7a水平与淋巴结转移有关,有助于肿瘤病情判断与术后病情监测,但有待进一步研究。
中图分类号:
1 | 陈文杰, 王斌全, 高伟, 等 . 喉癌流行病学特征及影响因素分析[J]. 中国当代医药, 2015, 22(12): 43-46. |
CHEN Wenjie , WANG Binquan , GAO Wei , et al . Analysis of epidemiologic features and influence factors of carcinoma of larynx[J]. China Modern Medicine, 2015, 22(12): 43-46. | |
2 |
Olaleye O , Siddiq S , Ekrikpo U , et al . Regional differences in incidence and mortality trends in cancers of the larynx, thyroid, oral cavity and pharynx in England and Scotland: 1975-2002[J]. OJEpi, 2013, 3(2): 70-78. doi: 10.4236/ojepi.2013.32011 .
doi: 10.4236/ojepi.2013.32011 |
3 |
李文明,魏东敏,钱晔,等 .支撑喉镜下CO_2激光治疗喉癌的疗效分析[J].山东大学耳鼻喉眼学报,2018,32(6):13-17.doi:10.6040/j.issn.1673-3770.1.2018.025 .
doi: 10.6040/j.issn.1673-3770.1.2018.025 |
LI Wenming , WEI Dongmin , QIAN Ye , et al . Evaluate the curative effect of CO2 laser in treatment of laryngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(6): 13-17. doi:10.6040/j.issn.1673-3770.1.2018.025 .
doi: 10.6040/j.issn.1673-3770.1.2018.025 |
|
4 |
Forastiere AA , Weber RS , Trotti A . Organ Preservation for Advanced Larynx Cancer: Issues and Outcomes[J]. Journal of Clinical Oncology, 2015, 33(29):3262-3268.doi:10.1200/jco.2015.61.2978 .
doi: 10.1200/jco.2015.61.2978 |
5 |
Chen CC , You JY , Lung J , et al . Aberrant let7a/HMGA2 signaling activity with unique clinical phenotype in JAK2 -mutated myeloproliferative neoplasms[J]. Haematologica, 2017, 102(3): 509-518. doi:10.3324/haematol.2016.154385 .
doi: 10.3324/haematol.2016.154385 |
6 |
Xu CW , Sun X , Qin SD , et al . Let-7a regulates mammosphere formation capacity through Ras/NF-κB and Ras/MAPK/ERK pathway in breast cancer stem cells[J]. Cell Cycle, 2015, 14(11): 1686-1697. doi:10.1080/15384101.2015.1030547 .
doi: 10.1080/15384101.2015.1030547 |
7 |
Chen CC , You JY , Gau JP , et al . Favorable clinical outcome and unique characteristics in association with Twist1 overexpression in de novo acute myeloid leukemia[J]. Blood Cancer J,2015,5:e339. doi: 10.1038/bcj. 2015.67 .
doi: 10.1038/bcj. 2015.67 |
8 |
Deng MM , Hou JW , Hu J , et al . Hepatitis B virus mRNAs functionally sequester let-7a and enhance hepatocellular carcinoma[J]. Cancer Lett, 2016, 383(1): 62-72. doi:10.1016/j.canlet.2016.09.028 .
doi: 10.1016/j.canlet.2016.09.028 |
9 |
马丽娟, 刘金华, 肖旭平 . 喉癌组织miRNAlet-7a和HMGA2表达临床意义研究[J]. 中华肿瘤防治杂志, 2016, 23(2): 74-78. doi:10.16073/j.cnki.cjcpt.2016.02. 006 .
doi: 10.16073/j.cnki.cjcpt.2016.02.006 |
MA Lijuan , LIU Jinhua , XIAO Xuping . Clinical significance of expressions of miRNA let-7a and HMGA2 in laryngeal squamous cell carcinoma tissues[J]. Chinese Journal of Cancer Prevention and Treatment, 2016, 23(2): 74-78. doi:10.16073/j.cnki.cjcpt.2016.02.006 .
doi: 10.16073/j.cnki.cjcpt.2016.02.006 |
|
10 | 尹娟 . Let-7a在喉癌细胞中调控HMGA2的机制研究[D]. 长沙: 湖南师范大学, 2017. |
11 | 周恩 . let-7a调控HMGA2对喉癌Hep-2细胞裸鼠移植瘤生长的影响及其机制[D]. 长沙:湖南师范大学, 2017:34-77. |
12 |
Petriella D , De Summa S , Lacalamita R , et al . miRNA profiling in serum and tissue samples to assess noninvasive biomarkers for NSCLC clinical outcome[J]. Tumour Biol, 2016, 37(4): 5503-5513. doi:10.1007/s13277-015-4391-1 .
doi: 10.1007/s13277-015-4391-1 |
13 |
Witwer KW . Circulating microRNA biomarker studies: pitfalls and potential solutions[J]. Clin Chem, 2015, 61(1): 56-63. doi:10.1373/clinchem.2014.221341 .
doi: 10.1373/clinchem.2014.221341 |
14 |
Chen WD , Li SJ . Circulating microRNA as a novel biomarker for pulmonary arterial hypertension due to congenital heart disease[J]. Pediatr Cardiol, 2017, 38(1): 86-94. doi:10.1007/s00246-016-1487-3 .
doi: 10.1007/s00246-016-1487-3 |
15 |
Hu ZB , Chen X , Zhao Y , et al . Serum microRNA signatures identified in a genome-wide serum microRNA expression profiling predict survival of non-small-cell lung cancer[J]. J Clin Oncol, 2010, 28(10): 1721-1726. doi:10.1200/JCO.2009.24.9342 .
doi: 10.1200/JCO.2009.24.9342 |
16 |
Huang YR , Yu S , Cao ST , et al . MicroRNA-222 promotes invasion and metastasis of papillary thyroid cancer through targeting protein phosphatase 2 regulatory subunit b alpha expression[J]. Thyroid, 2018, 28(9): 1162-1173. doi:10.1089/thy.2017.0665 .
doi: 10.1089/thy.2017.0665 |
17 |
Wang CL , Wang D , Yan BZ , et al . Long non-coding RNA NEAT1 promotes viability and migration of gastric cancer cell lines through up-regulation of microRNA-17[J]. Eur Rev Med Pharmacol Sci, 2018, 22(13): 4128-4137. doi:10.26355/eurrev_201807_15405 .
doi: 10.26355/eurrev_201807_15405 |
18 |
Li Y , Dai Y , Zhang XJ , et al . Three-layered polyplex as a microRNA targeted delivery system for breast cancer gene therapy[J]. Nanotechnology, 2017, 28(28): 285101. doi:10.1088/1361-6528/aa757f .
doi: 10.1088/1361-6528/aa757f |
19 |
Hojo N , Hill A , Chirshev E , et al . Mechanism of tumor suppressor miRNA let-7 downregulation in ovarian cancer: transcription factor Snail represses let-7 and is associated with invasiveness phenotype[J]. Gynecologic Oncology, 2017, 147(1): 194. doi:10.1016/j.ygyno. 2017. 07.022 .
doi: 10.1016/j.ygyno. 2017. 07.022 |
20 |
Liu WJ , Xu Q , Sun LP , et al . Expression of serum let-7c, let-7i, and let-7f microRNA with its target gene, pepsinogen C, in gastric cancer and precancerous disease[J]. Tumour Biol, 2015, 36(5): 3337-3343. doi:10.1007/s13277-014-2967-9 .
doi: 10.1007/s13277-014-2967-9 |
[1] | 申宇鹏,宋琦,李晓明. 喉癌前病变的病因、分子机制和处理策略[J]. 山东大学耳鼻喉眼学报, 2019, 33(4): 25-30. |
[2] | 徐夏,邓文婷,黄成志,陈勇挺,汪建. 喉内镜下等离子消融术与开放性手术治疗早期喉癌的疗效对比[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 81-85. |
[3] | 黄志刚. CO2激光手术治疗咽喉部肿瘤[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 1-3. |
[4] | 刘鸣. 声门上型喉癌的CO2激光治疗[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 4-7. |
[5] | 李文明,魏东敏,钱晔,曹晟达,许雅,刘大昱,潘新良,雷大鹏. 支撑喉镜下CO2激光治疗喉癌的疗效分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 13-17. |
[6] | 许莉,王志颐,陈伟,薛飞,张勇,程友,季俊峰,崔鹏程,江满杰. MicroRNA-24对人喉鳞癌细胞生物学行为的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 30-37. |
[7] | 叶树凤,张旭,苏凯,李贺杰,滕博. 喉癌喉部分切除术术后喉囊肿一例及文献复习[J]. 山东大学耳鼻喉眼学报, 2018, 32(5): 117-118. |
[8] | 李国彬,张占成,王新颜. 上调miR-200b对人喉癌Hep-2细胞增殖、迁移和侵袭能力的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 53-57. |
[9] | 浦洪波,杜晓东. 无锡地区2 000例变应性鼻炎变应原检测结果分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 105-107. |
[10] | 邓月,徐亚平,彭浒,陶绮蕾,范静平,孙爱华,吴建. 铁必复颗粒治疗缺铁感音神经性聋患者的疗效分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(1): 75-79. |
[11] | 金成勋,赵雪,刘悦,魏宁,金春顺. Kimura病11例并文献复习[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 63-66. |
[12] | 马玲国,郑朝攀,周敬淳,张博,龚桃根,张云静. CO2激光治疗声门型喉癌远期疗效分析[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 98-100. |
[13] | 包伟晶,宁佳羽,朱忠寿,魏日富,林昶. CO2激光切除累及前联合的早期声门癌26例[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 101-105. |
[14] | 许元腾,陈瑞庆,林功标,方秀玲,俞舒娟,梁晓华,张榕. RNAi沉默PDCD4基因对人喉癌Hep-2细胞增殖及β-catenin表达的影响[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 110-114. |
[15] | 邰旭辉,杨昕,朱宝玉,曹鋐,褚彦玲,单晓东,姜宇. 辽宁地区2 883例变应性鼻炎患者变应原分布特征[J]. 山东大学耳鼻喉眼学报, 2016, 30(4): 39-42. |
|