山东大学耳鼻喉眼学报 ›› 2021, Vol. 35 ›› Issue (3): 81-86.doi: 10.6040/j.issn.1673-3770.0.2020.016

• 临床研究 • 上一篇    下一篇

鼻咽癌患者miR-429、miR-200C表达与预后关系分析

王中卫,杨林,郭亚,孙斌,王亚利,马秀龙,任宏涛,包兴   

  1. 西安交通大学第二附属医院 放疗科, 陕西 西安 710004
  • 发布日期:2021-05-14
  • 通讯作者: 王中卫. E-mail:wangzhongwei601@163.com

Analysis of the relationship between miR-429 and miR-200C expression and prognosis in patients with nasopharyngeal carcinoma

WANG Zhongwei, YANG Lin, GUO Ya, SUN Bin, WANG Yali, MA Xiulong, REN Hongtao, BAO Xing   

  1. Department of Radiation Therapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China
  • Published:2021-05-14

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摘要： 目的 探讨鼻咽癌患者miR-429、miR-200C表达与预后关系。 方法 选择2016年1月至2017年5月接受治疗的初治鼻咽癌患者80例,对病理穿刺组织样本行qRT-PCR检测对miR-429、miR-200C相抵表达量进行分析,治疗3个月后进行疗效评价。出院后以电话及门诊复诊的方式进行随访,记录患者生存时间。 结果 不同病理分期、T分期、N分期及临床分期患者miR-429及miR-200C相对表达量差异均具有统计学意义,且均随严重程度的增加表达量下降(P<0.05)。治疗3个月后80例患者总体有效率为100%,其中CR 71例(88.75%),PR 9例(11.25%)。死亡及复发或转移的55例为预后不良组,无瘤生存的18例为预后良好组,预后良好组患者miR-429及miR-200C相对表达量均高于预后不良组(P<0.05)。miR-429相对表达量对鼻咽癌预后不良预测cut-off值为1.36,AUC为0.723,95%CI为0.547~0.898,灵敏度为82.15%,特异度为81.72%;miR-200C相对表达量对鼻咽癌预后不良预测cut-off值为0.53,AUC为0.713,95%CI为0.562~0.865,灵敏度为82.01%,特异度为79.61%。miR-429高表达组(miR-429相对表达量≥1.36)平均生存时间为(39.07±1.59)个月,95%CI:35.95~42.19;miR-429低表达组(miR-429相对表达量<1.36)平均生存时间为(23.57±1.02)个月,95%CI:21.57~25.58(P<0.05)。miR-200C高表达组(miR-200C相对表达量≥0.53)平均生存时间为(38.28±1.71)个月,95%CI:34.94~41.63;miR-200C低表达组(miR-200C相对表达量<0.53)平均生存时间为(23.52±1.07)个月,95%CI:21.42~25.62(P<0.05)。 结论 不同病理分期、T分期、N分期及临床分期鼻咽癌患者miR-429、miR-200C相对表达量有差异,且随着病情严重程度的升高miR-429、miR-200C表达量下降,miR-429相对表达量<1.36、miR-200C相对表达量<0.53时鼻咽癌患者生存时间下降。

关键词: 鼻咽癌, miR-429, miR-200C, 预后, 生存分析

Abstract: Objective To investigate the relationship between the expression of miR-429, miR-200C and prognosis in patients with nasopharyngeal carcinoma. Methods 80 patients with nasopharyngeal carcinoma who received treatment between January 2016 and May 2017 were selected Tissue puncture samples were subjected to qRT-PCR detection to analyze the offset expression of miR-429 and miR-200C, and three treatments were performed. The Efficacy will be evaluated in the coming months. After discharge from the hospital, follow-up was conducted by telephone and outpatient follow-up, and the survival time of the patient was recorded. Results The relative expression levels of miR-429 and miR-200C in patients with different pathological stages, T stages, N stages and clinical stages were statistically significantly different, and the expression levels decreased with the increase of severity(P<0.05). After three months of treatment, the overall effective rate of 80 patients was 100%, of which 71 were CR(88.75%)and 9 were PR(11.25%). Fifty-five patients with death, recurrence or metastasis belonged to the poor prognosis group, and 18 patients with tumor-free survival belonged to the good prognosis group. The relative expression levels of miR-429 and miR-200C in the good prognosis group were higher than those in the poor prognosis group(P<0.05). The relative expression of miR-429 has a predictive cut-off value of 1.36 for the poor prognosis of nasopharyngeal carcinoma. The AUC was 0.723, 95% CI of 0.547 to 0.898, sensitivity of 82.15%, and the specificity was 81.72%; relative expression of miR-200C The predictive cut-off value of poor prognosis for nasopharyngeal carcinoma was 0.53, the AUC was 0.713, 95%CI was 0.562-0.865, the sensitivity was 82.01%, and the specificity was 79.61%. The average survival time of the miR-429 high expression group(miR-429 relative expression level ≥ 1.36)was 39.07±1.59 months, with a 95%CI: of 35.95-42.19; miR-429 in the low expression group(miR-429 relative expression level < 1.36). The average survival time was 23.57±1.02 months, with a 95%CI of 21.57-25.58(P<0.05). The average survival time of the miR-200C high expression group(miR-200C relative expression ≥0.53)was(38.28±1.71)months the 95%CI was 34.94-41.63; and the miR was-200C in the low expression group(miR-200C relative expression <0.53 )The average survival time was 23.52±1.07 months, 95%CI: 21.42-25.62(P<0.05). Conclusion The relative expression levels of miR-429 and miR-200C in nasopharyngeal carcinoma patients at different pathological stages, T stages, N stages and clinical stages were different, and the expression levels of miR-429 and miR-200C decreased as the severity of the disease increased. The survival time of nasopharyngeal carcinoma patients decreased when the relative expression of miR-429 was <1.36 and the relative expression of miR-200C was <0.53.

Key words: Nasopharyngeal carcinoma, miR-429, miR-200C, Prognosis, Survival analysis

中图分类号: 

  • R766.04
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