山东大学耳鼻喉眼学报 ›› 2021, Vol. 35 ›› Issue (1): 30-34.doi: 10.6040/j.issn.1673-3770.0.2020.127

• • 上一篇    下一篇

E-cadherin和ZEB2在鼻腔鼻窦内翻性乳头状瘤中的表达及其临床意义

石书婧,方静蕾,张安琪,徐莉,刘冉冉,韩振强,王建国   

  1. 河北省眼科医院 耳鼻咽喉头颈外科, 河北 邢台 054000
  • 发布日期:2021-02-01
  • 通讯作者: 王建国. E-mail:wjg202712@sina.com
  • 基金资助:
    邢台市科技局支撑项目(2016ZC259)

Expressions of E-cadherin and ZEB2 in sinonasal inverted papilloma and their clinical significance

SHI Shujing, FANG Jinglei, ZHANG Anqi, XU Li, LIU Ranran, HAN Zhenqiang, WANG Jianguo   

  1. Department of Otolaryngology & Head and Neck Surgery, Hebei Eye Hospital, Xingtai 054000, Hebei, China
  • Published:2021-02-01

摘要: 目的 探讨E-cadherin mRNA和ZEB2 mRNA在鼻腔鼻窦内翻性乳头状瘤(SNIP)中的表达及其临床意义。 方法 采用荧光定量PCR法检测E-cadherin mRNA和ZEB2 mRNA在51例SNIP和20例增生鼻甲组织中的表达,探讨E-cadherin mRNA和ZEB2 mRNA表达与SNIP患者临床病理参数之间的关系以及两者之间的相关性。 结果 (1) E-cadherin mRNA在SNIP中的表达水平低于增生鼻甲组织(P=0.007),ZEB2 mRNA在SNIP中的表达水平高于增生鼻甲组织(P=0.034),差异均具有统计学意义;(2) E-cadherin mRNA的低表达和ZEB2 mRNA的高表达与SNIP年龄、性别、临床分期及是否复发均无明显相关性,而与SNIP病理分化程度有关,其中在无不典型增生组与恶变组之间的表达差异具有统计学意义;(3) E-cadherin mRNA和ZEB2 mRNA的表达呈负相关(r=-0.317, P=0.023)。 结论 在SNIP中 E-cadherin mRNA表达下调、ZEB2 mRNA表达上调,两者的异常表达呈负相关,且均与SNIP病理分化程度有关,其中在无不典型增生组与恶变组之间的表达差异均具有统计学意义,ZEB2 mRNA可能通过下调E-cadherin mRNA的表达进而促进SNIP的发生及恶变。

关键词: E-cadherin, ZEB2, 鼻腔鼻窦内翻性乳头状瘤, 病理分化程度, 发生, 恶变

Abstract: Objective To investigate the expressions of E-cadherin and ZEB2 mRNAs in sinonasal inverted papilloma(SNIP)and assess their clinical significance. Methods Quantitative fluorescence polymerase chain reaction was performed to detect the expressions of E-cadherin and ZEB2 mRNAs in 51 cases of SNIP and 20 cases of hyperplastic turbinate tissues and investigate the relationship and correlation between E-cadherin and ZEB2 mRNA expressions and clinicopathological parameters of SNIP patients. Results The expression of E-cadherin mRNA was lower in SNIP than in hyperplastic turbinate tissue(P=0.007)and that of ZEB2 mRNA was higher in SNIP than in hyperplastic turbinate tissue(P=0.034), both with statistically significant differences. The low expression of E-cadherin mRNA and high expression of ZEB2 mRNA were not related to the age, sex, clinical stage, or recurrence of SNIP, but were related to the pathological differentiation of SNIP. Further, the difference between the atypical hyperplasia and malignant transformation groups was statistically significant. The expressions of E-cadherin and ZEB2 mRNAs correlated negatively(r=-0.317, P=0.023). Conclusion In SNIP, the E-cadherin mRNA expression was downregulated, whereas ZEB2 mRNA expression was upregulated, showing a negative correlation. Both abnormal expressions were related to the pathologic differentiation of SNIP. The mechanism may be downregulation of the E-cadherin expression by ZEB2, promoting the occurrence and malignant transformation of SNIP.

Key words: E-cadherin, ZEB2, Sinonasal inverted papilloma, Degree of pathological differentiation, Occurrence, Malignant transformation

中图分类号: 

  • R739.62
[1] Fang GL, Lou HF, Yu WL, et al. Prediction of the originating site of sinonasal inverted Papilloma by preoperative magnetic resonance imaging and computed tomography[J]. Int Forum Allergy Rhinol, 2016, 6(12):1221-1228. doi:10.1002/alr.21836.
[2] Nowosielska-Grygiel J, Pietkiewicz P, Owczarek K, et al. Diagnosis and treatment of nasal and paranasal inverted papillomas-epidemiology and own experience[J]. Otolaryngol Pol, 2017, 71(6):27-32. doi:10.5604/01.3001.0010.7195.
[3] Akkari M, Lassave J, Mura T, et al. Atypical presentations of sinonasal inverted papilloma: Surgical management and influence on the recurrence rate[J]. Am J Rhinol Allergy, 2016, 30(2):149-154. doi:10.2500/ajra.2016.30.4288.
[4] Lu W, Kang YB. Epithelial-mesenchymal plasticity in cancer progression and metastasis[J]. Dev Cell, 2019, 49(3):361-374. doi:10.1016/j.devcel.2019.04.010.
[5] Dongre A, Weinberg RA. New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer[J]. Nat Rev Mol Cell Biol, 2019, 20(2):69-84. doi:10.1038/s41580-018-0080-4.
[6] 王艳杰, 耿志刚, 赵长青, 等. 鼻腔鼻窦内翻性乳头状瘤的临床分期及术式选择[J]. 中国中西医结合耳鼻咽喉科杂志, 2020, 28(1):75-79. doi:10.16542/j.cnki.issn.1007-4856.2020.01.020. WANG Yanjie, GENG Zhigang, ZHAO Changqing, et al. The clinical classification systems and surgical methods for sinonasal inverted Papilloma[J]. Chinese Journal of Otorhinolaryngology in Integrative Medicine, 2020, 28(1):75-79. doi:10.16542/j.cnki.issn.1007-4856.2020.01.020.
[7] 王愿, 周涵, 刘晓静, 等. 鼻内翻性乳头状瘤的术式选择和疗效分析[J]. 山东大学耳鼻喉眼学报, 2018, 32(2):79-83. doi:10.6040/j.issn.1673-3770.0.2017.441. WANG Yuan, ZHOU Han, LIU Xiaojing, et al. Surgical strategies for nasal inverted Papilloma and corresponding recurrence rates[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(2):79-83. doi:10.6040/j.issn.1673-3770.0.2017.441.
[8] Coutinho G, Marques J, Leal M, et al. Surgical outcomes of sinonasal inverted papilloma: a 17 year review[J]. Braz J Otorhinolaryngol, 2020, 86(3):315-320. doi:10.1016/j.bjorl.2018.12.011.
[9] Udager AM, McHugh JB, Goudsmit CM, et al. Human papillomavirus(HPV)and somatic EGFR mutations are essential, mutually exclusive oncogenic mechanisms for inverted sinonasal papillomas and associated sinonasal squamous cell carcinomas[J]. Ann Oncol, 2018, 29(2):466-471. doi:10.1093/annonc/mdx736.
[10] Derynck R, Weinberg RA. EMT and cancer: more than meets the eye[J]. Dev Cell, 2019, 49(3):313-316. doi:10.1016/j.devcel.2019.04.026.
[11] Jeanes A, Gottardi CJ, Yap AS. Cadherins and cancer: how does cadherin dysfunction promote tumor progression?[J]. Oncogene, 2008, 27(55):6920-6929. doi:10.1038/onc.2008.343.
[12] Huang RYJ, Guilford P, Thiery JP. Early events in cell adhesion and polarity during epithelial-mesenchymal transition[J]. J Cell Sci, 2012, 125(19):4417-4422. doi:10.1242/jcs.099697.
[13] Webber HC, Bermudez JY, Millar JC, et al. The role of wnt/β-catenin signaling and K-cadherin in the regulation of intraocular pressure[J]. Invest Ophthalmol Vis Sci, 2018, 59(3):1454. doi:10.1167/iovs.17-21964.
[14] 杜刚, 黄瑛. 膀胱癌组织中PVRL4、NF-κB p65、E-cadherin的表达变化及意义[J]. 山东医药, 2019, 59(28):29-32. doi:10.3969/j.issn.1002-266X.2019.28.007. DU Gang, HUANG Ying. Expression and clinical significance of PVRL4, NF-κB p65 and E-cadherin in bladder cancer tissues[J]. Shandong Medical Journal, 2019, 59(28):29-32. doi:10.3969/j.issn.1002-266X.2019.28.007.
[15] 谭红, 毛亮, 王敏, 等. E-钙黏素mRNA在大鼠舌黏膜癌变过程中的表达研究[J]. 口腔医学研究, 2016, 32(1):31-34. doi:10.11839/kqyxzh.2016.01.008. TAN Hong, MAO Liang, WANG Min, et al. expression of E-cadherin mRNA in the rat tongue carcinogenesis[J]. Journal of Oral Science Research, 2016, 32(1):31-34. doi:10.11839/kqyxzh.2016.01.008.
[16] 孙菊杰, 魏玲, 张德贤, 等. 非小细胞肺癌中Snail、HIF-1α和E-cadherin 表达的临床意义及相关性[J]. 肿瘤防治研究, 2015, 42(1):37-40. doi:10.3971/j.issn.1000-8578.2015.01.009. SUN Jujie, WEI Ling, ZHANG Dexian, et al. Clinical significance of snail, hypoxia-induced factor-1α and E-cadherin expression in non-small cell lung cancer and their correlations[J]. Cancer Research on Prevention and Treatment, 2015, 42(1):37-40. doi:10.3971/j.issn.1000-8578.2015.01.009.
[17] Olson A, Le V, Aldahl J, et al. The comprehensive role of E-cadherin in maintaining prostatic epithelial integrity during oncogenic transformation and tumor progression[J]. PLoS Genet, 2019, 15(10):e1008451. doi:10.1371/journal.pgen.1008451.
[18] Goossens S, Wang J, Tremblay CS, et al. ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms[J]. Haematologica, 2019, 104(8):1608-1616. doi:10.3324/haematol.2018.207837.
[19] DaSilva-Arnold SC, Kuo CY, Davra V, et al. ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation[J]. Mol Hum Reprod, 2019, 25(2):61-75. doi:10.1093/molehr/gay053.
[20] Li Y, Zhang JL, Zhang W, et al. MicroRNA-192 regulates hypertrophic scar fibrosis by targeting SIP1[J]. J Mol Histol, 2017, 48(5/6):357-366. doi:10.1007/s10735-017-9734-3.
[21] Ji H, Sang MX, Liu F, et al. miR-124 regulates EMT based on ZEB2 target to inhibit invasion and metastasis in triple-negative breast cancer[J]. Pathol-Res Pract, 2019, 215(4):697-704. doi:10.1016/j.prp.2018.12.039.
[22] Suzuki K, Kawataki T, Endo K, et al. Expression of ZEBs in gliomas is associated with invasive properties and histopathological grade[J]. Oncol Lett, 2018, 16(2):1758-1764. doi:10.3892/ol.2018.8852.
[23] Li XT, Li CH, Bi HM, et al. Targeting ZEB2 by microRNA-129 in non-small cell lung cancer suppresses cell proliferation, invasion and migration via regulating wnt/β-catenin signaling pathway and epithelial-mesenchymal transition[J]. Onco Targets Ther, 2019, 12:9165-9175. doi:10.2147/OTT.S217536.
[24] Chen XF, Guo JF, Xu JF, et al. MiRNA-206 inhibits proliferation of renal clear cell carcinoma by targeting ZEB2[J]. Eur Rev Med Pharmacol Sci, 2019, 23(18):7826-7834. doi:10.26355/eurrev_201909_18992.
[25] Hu B, Sun M, Liu J, et al. MicroRNA-204 suppressed proliferation and motility capacity of human hepatocellular carcinoma via directly targeting zinc finger E-box binding homeobox 2[J]. Oncol Lett, 2017, 13(5):3823-3830. doi:10.3892/ol.2017.5907.
[26] Kan QE, Su Y, Yang HH. MicroRNA-335 is downregulated in papillary thyroid cancer and suppresses cancer cell growth, migration and invasion by directly targeting ZEB2[J]. Oncol Lett, 2017, 14(6):7622-7628. doi:10.3892/ol.2017.7126.
[1] 马静远, 武天义, 孙占伟, 王卫卫, 李世超, 王广科. 鼻腔鼻窦内翻性乳头状瘤与外周血炎症标志物的相关性研究[J]. 山东大学耳鼻喉眼学报, 2022, 36(4): 35-39.
[2] 李祯,崔丽梅,孙岩. 骨形态发生蛋白4在内耳发育及在毛细胞与螺旋神经节细胞再生中的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(2): 108-112.
[3] 吕静荣,陈淳,马衍,谢晋. 儿童分化型甲状腺癌的临床特征及危险因素分析[J]. 山东大学耳鼻喉眼学报, 2020, 34(3): 88-94.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 张晗,黄一飞 . 抗角膜移植排斥的研究进展[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 84 -87 .
[2] 牛善利,柴茂文,李振秀 . 鼻内镜下鼻甲成形术治疗慢性肥厚性鼻炎60例[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 16 -18 .
[3] 孟庆国,卢永田,范献良 . 杀伤细胞免疫球蛋白样受体基因多态性与鼻咽癌的关联性[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 196 -199 .
[4] 马玉起,孔祥春 . 先天性双侧下唇窦道1例[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 199 -199 .
[5] 万俐佳,鲁海涛,姜义道,刘 辉,李 琴,佘腊枝 . 改良腭咽成形术治疗阻塞性睡眠呼吸暂停综合征41例[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 204 -205 .
[6] 于青青 ,王跃建 . 硬质耳内镜的临床应用进展[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 222 -224 .
[7] 吉晓滨,邓家德,臧林泉,王 磊,谢 军 . 豚鼠变应性鼻炎模型血清组胺的测定[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 228 -230 .
[8] 向登,卢永田,孙焕吉 . 鼻内镜下修补脑脊液鼻漏19例并文献复习[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 234 -236 .
[9] 邱恩惠,李志春,方文旭 . 嗅觉障碍的中西医治疗[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 253 -257 .
[10] 殷国华,钟 笑 . 激光减容术治疗舌扁桃体肥大的远期疗效[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 280 -282 .