山东大学耳鼻喉眼学报 ›› 2022, Vol. 36 ›› Issue (2): 32-39.doi: 10.6040/j.issn.1673-3770.0.2021.119

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CHD1L通过EMT促进喉鳞状细胞癌细胞的增殖、侵袭和转移

刘勇1,袁存立1,曹慧1,郑成彩1,晁方2, 许风雷3   

  1. 1.日照市中心医院 耳鼻喉科, 山东 日照 276800;
    2.日照市人民医院 第一手术室, 山东 日照 276826;
    3.山东省立医院 耳鼻喉科, 山东 济南 250022
  • 发布日期:2022-04-15
  • 通讯作者: 许风雷. E-mail:xufenglei@sdu.edu.cn
  • 基金资助:
    山东省重点研发项目基金(2018GSF118091)

CHD1L promotes proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells by EMT

LIU Yong1, YUAN Cunli1, CAO Hui1, ZHENG Chengcai1, CHAO Fang2, XU Fenglei3   

  1. 1.Department of Otolaryngology, Rizhao Central Hospital, Rizhao 276800, Shandong, China;
    2.First Operating Room, Rizhao People's Hospital, Rizhao 276826, Shandong, China;
    3. Department of Otolaryngology, Shandong Provincial Hospital, Jinan 250022, Shandong, China
  • Published:2022-04-15

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摘要： 目的 探讨染色质解旋酶DNA结合蛋白(CHD1L)在喉鳞状细胞癌(LSCC)增殖、侵袭和转移中的作用机制,为喉鳞状细胞癌的治疗提供分子靶点。 方法 免疫组织化学方法检测60例喉鳞状细胞癌组织和其配对癌旁正常黏膜组织中CHD1L、上皮性钙黏着蛋白(E-cadherin)和波形蛋白(Vimentin)的表达;Western blotting法检测人支气管上皮细胞(HBE)和人喉表皮样癌细胞(HEp-2)中CHD1L表达。敲除HEp-2细胞中的CHD1L,并用Western blotting法检测HEp-2细胞内CHD1L、E-cadherin和Vimentin的表达;CCK-8实验检测各组细胞增殖情况,Transwell侵袭实验检测各组细胞的侵袭情况。 结果 免疫组化结果显示CHD1L在喉鳞状细胞癌组织中的阳性表达率明显高于癌旁正常黏膜组织中的阳性表达率,且其阳性表达与喉鳞状细胞癌的分化程度、淋巴结转移、临床分期及Vimentin的表达呈正相关,而与E-cadherin的表达呈负相关(P<0.05)。Western blotting结果显示CHD1L在HEp-2细胞中的表达明显高于HBE细胞。用干扰质粒体外敲减HEp-2细胞中的CHD1L。体外敲减CHD1L的表达水平之后,HEp-2细胞的增殖能力及侵袭能力明显降低,E-cadherin的表达明显升高,Vimentin的表达明显降低。 结论 喉鳞状细胞癌组织中CHD1L呈高表达,与喉鳞状细胞癌细胞分化程度、临床分期及淋巴结转移密切相关,提示CHD1L在喉鳞状细胞癌中起着癌基因的作用;CHD1L可以通过促进喉鳞状细胞癌细胞的EMT进而促进喉鳞状细胞癌细胞的增殖、侵袭和转移。

关键词: 喉鳞状细胞癌, 染色质解旋酶DNA结合蛋白, 上皮-间充质转化, 增殖, 侵袭, 转移

Abstract: Objective To investigate the mechanism of chromodomain helicase DNA binding protein 1-like gene(CHD1L )in the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells and to provide molecular targets for the treatment of laryngeal squamous cell carcinoma(LSCC). Methods Immunohistochemistry was conducted to measure the expression of CHD1L, E-cadherin and Vimentin in 60 pairs of LSCC and its adjacent normal mucosa. The expression of CHD1L in human bronchial epithelial cell(HBE)and human laryngeal epithelioid carcinoma cell(HEp-2)was detected by Western blotting. CHD1L in HEp-2 cells was knocked out, and Western blotting was used to detect the expression of CHD1L, E-cadherin and Vimentin in HEp-2 cells. The proliferation of cells in each group was detected by CCK-8 kit, and the invasion of cells in each group was detected by Transwell invasion test. Results Immunohistochemical results showed that the positive expression rate of CHD1L in laryngeal squamous cell carcinoma tissue was significantly higher than that of normal paracaner mucosa tissues. In addition, the expression of CHD1L was positively correlated with the degree of differentiation of laryngeal squamous cell carcinoma, the lymph node metastasis, the clinical stage and the expression of Vimentin, but negatively correlated with the expression of E-cadherin(P<0.05). The results of Western blotting showed that the expression of CHD1L in HEp-2 cell was significantly higher than that in HBE cell. CHD1L in HEp-2 cells was knocked down by interfering plasmid in vitro. The proliferation and invasion ability of the HEp-2 cells with CHD1L knockdown in vitro were significantly decreased, while the expression of E-cadherin was significantly increased and the expression of Vimentin was also significantly decreased. Conclusion The high expression of CHD1L in LSCC was closely related to pathological grades, clinical stage and risk of lymph node metastasis of LSCC, suggesting that CHD1L may play an oncogene role in LSCC; CHD1L could promote the proliferation, invasion and metastasis of LSCC cells by promoting the EMT of LSCC cells.

Key words: Laryngeal squamous cell carcinoma, Chromodomain helicase/ATPase DNA binding protein 1-like gene, Epithelial-mesenchymal transition, Proliferation, Invasion, Metastasis

中图分类号: 

  • R739.65
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