山东大学耳鼻喉眼学报 ›› 2023, Vol. 37 ›› Issue (1): 41-46.doi: 10.6040/j.issn.1673-3770.0.2021.502
• 论著 • 上一篇
黄恒丰1,2,马鲲鹏2,杨迪1,2,张丽君2,张盛林3
HUANG Hengfeng1,2, MA Kunpeng2, YANG Di1,2, ZHANG Lijun2, ZHANG Shenglin3
摘要: 目的 miR-181b-5P和红细胞膜蛋白配体4.1样3(EPB41L3)目前在喉癌中表达关系及作用机制尚不明确。论文主要通过研究miR-181b-5P、EPB41L3蛋白在喉癌组织及癌旁正常组织中表达水平并进行相关性分析,探讨miR-181b-5P靶向调控EPB41L3对喉癌组织增殖、侵袭及转移的影响。 方法 通过生物信息学数据库预测miR-181b-5P在喉癌中的表达情况,采用实时荧光定量PCR技术测定喉癌及癌旁正常组织中miR-181b-5P的表达量。预测miR-181b-5p的靶基因并进行生物信息学分析,EPB41L3蛋白的相对表达由免疫组化SP法测定。 结果 喉癌组织中miR-181b-5P的表达明显较癌旁正常组织高(P<0.001),且在有无淋巴结转移间有显著差异(P=0.027 3)。EPB41L3蛋白在喉癌组织的表达明显较癌旁正常组织低(P<0.001)。EPB41L3蛋白在喉癌中的阳性表达率于不同病理分化程度、临床分期及有无淋巴结转移中差异具有统计学意义(P<0.05)。miR-181b-5p及EPB41L3蛋白在喉癌组织中的表达呈负相关(r=-0.420 8,P=0.002 3)。 结论 miR-181b-5P可能通过负性调控EPB41L3的表达,影响喉癌组织的增殖、侵袭、转移,研究可为喉癌的临床靶向治疗提供新的思路。
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