Abstract:

Objective    To investigate the effect of ultraviolet b (UVB) on glycoprotein nonmetastatic melanoma protein b (GPNMB) in human retinal pigment epithelium (RPE) cells. Methods    MTT assay was used to determine the effect of UVB on RPE cell survival rate. The effect of UVB on GPNMB in RPE cells was detected by RT-PCR and western blot. Results    MTT assay showed that RPE cells were damaged by UVB irradiation at 100 μW/cm2 for 2 h and the number of apoptotic cells significantly increased. The results of RT-PCR and western blot demonstrated that the expression of GPNMB in RPE cells markedly enhanced after UVB irradiation. Conclusion    UVB irradiation could suppress the survival rate of RPE cells and promote the expression of GPNMB in RPE cells.

Key words: Glycoprotein nonmetastatic melanoma protein b, Ultraviolet b, Retinal pigment epithelium