JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (4): 69-73.doi: 10.6040/j.issn.1673-3770.0.2014.128

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Observation of collagen in PHEMA Keratoprothesis implanted into alkali burned rabbit corneas by picric sirius red staining.

BAI Hua1,2, GUO Huiling3, WANG Liqiang2, HUANG Yifei2   

  1. 1. Department of Ophthalmology, Beijing Command Military General Hospital, Beijing 100700, China;
    2. Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, China;
    3. Department of Ophthalmology, The 306th Hospital of PLA, Beijing 100853, China
  • Received:2014-04-22 Revised:2015-04-07 Online:2015-08-16 Published:2015-08-16

Abstract: Objective To observe the dynamic changes of type and distribution of collagen fiber in the porous skirt of new type one-piece keratoprosthesis (KPros) made of PHEMA implanted into the alkali burned rabbit corneas. Methods A total of 10 pieces KPros were implanted into the alkali burned rabbit corneas. The skirt of kpro was obtained after 2, 8, 16 and 22 weeks. The samples were treated with picric-sirius red staining and observed by polarizing microscopy. Results This material PHEMA allowed the cornea and surrounding tissue growing and forming a multi-site stable fiber-connections under polarized light after staining. The new organization within the pores was mainly type I collagen and its growth performance was that yellow-green type Ⅲ collagen become mature red type Ⅰ collagen in process and cells were active especially in limbus. Conclusion The dynamic changes of collagen observation, can be concluded that the PHEMA pores and the cornea shown the similar performance and consistent with normal rabbit corneal implant healing.

Key words: Polyhydroxyethyl methacrylate, Keratoprosthesis, Alkali burned, Picric sirius red staining, Polarizing microscope

CLC Number: 

  • R779.65
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[1] WANG Qun1, BAI Hua2, TIAN Lei2, WANG Li-qiang2, HUANG Yi-fei2. New soft one-piece PHEMA keratoprosthesis implanted into alkali burned rabbit corneas: an electron microscopy observation [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 59-62.
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