JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (4): 69-73.doi: 10.6040/j.issn.1673-3770.0.2014.128

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Observation of collagen in PHEMA Keratoprothesis implanted into alkali burned rabbit corneas by picric sirius red staining.

BAI Hua1,2, GUO Huiling3, WANG Liqiang2, HUANG Yifei2   

  1. 1. Department of Ophthalmology, Beijing Command Military General Hospital, Beijing 100700, China;
    2. Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, China;
    3. Department of Ophthalmology, The 306th Hospital of PLA, Beijing 100853, China
  • Received:2014-04-22 Revised:2015-04-07 Online:2015-08-16 Published:2015-08-16

Abstract: Objective To observe the dynamic changes of type and distribution of collagen fiber in the porous skirt of new type one-piece keratoprosthesis (KPros) made of PHEMA implanted into the alkali burned rabbit corneas. Methods A total of 10 pieces KPros were implanted into the alkali burned rabbit corneas. The skirt of kpro was obtained after 2, 8, 16 and 22 weeks. The samples were treated with picric-sirius red staining and observed by polarizing microscopy. Results This material PHEMA allowed the cornea and surrounding tissue growing and forming a multi-site stable fiber-connections under polarized light after staining. The new organization within the pores was mainly type I collagen and its growth performance was that yellow-green type Ⅲ collagen become mature red type Ⅰ collagen in process and cells were active especially in limbus. Conclusion The dynamic changes of collagen observation, can be concluded that the PHEMA pores and the cornea shown the similar performance and consistent with normal rabbit corneal implant healing.

Key words: Polyhydroxyethyl methacrylate, Keratoprosthesis, Alkali burned, Picric sirius red staining, Polarizing microscope

CLC Number: 

  • R779.65
[1] 白华, 高峰, 黄一飞, 等.新型PHEMA一体式人工角膜植入碱烧伤兔角膜的实验研究[J].解放军医学杂志,2003,28(12):1082-1083. BAI Hua, GAO Feng, HUANG Yifei, et al. Experimental study of one-piece PHEMA keratoprosthesis implantation in alkali injured cornea in rabbit[J]. Med J Chin People Liber Army, 2003, 28(12):1082-1083.
[2] 白华, 王丽强, 黄一飞. 新型一体式软性人工角膜支架植入碱烧伤兔角膜的组织病理学观察[J].中国组织工程研究, 2013, 17(34):6138-6143. BAI Hua, WANG Liqiang, HUANG Yifei. Histopathological observation of new soft one-piece keratoprosthesis skirt implanted into alkali burned rabbit corneas[J]. J Clin Rehab Tiss Eng Res, 2013, 17(34):6138-6143.
[3] 王群, 白华, 田磊, 等. 软性一体式PHEMA人工角膜植入碱烧伤兔角膜的电镜观察[J]. 山东大学耳鼻喉眼学报, 2014,28(3):59-62. WANG Qun, BAI Hua, TIAN Lei, et al. New soft one-piece PHEMA keratoprosthesis implanted into alkali burned rabbit corneas: an electron microscopy observation[J]. J Otolaryngol Ophthalmol Shandong Univ, 2014, 28(3):59-62.
[4] 蒙艳春, 高峰, 黄一飞,等.兔角膜植入改性HEMA人工角膜支架材料的实验研究[J].军医进修学院学报,2002,23(2):150-152. MENG Yanchun, GAO Feng, HUANG Yifei, et al. The experimental study of a moderated porous PHEMA material for the skirt of a keratoprosthesis[J]. Acad J Pla Postgrad Med School, 2002, 23(2):150-152.
[5] Gao F, Meng Y C, Qu B, et al. Preparation of artifical cornea with a central optic region and porous skirt[J]. Chin J Biomed Eng, 2002, 8(10):7-9.
[6] 胡平, 许波, 高峰. 一种用溶胀聚合粒子滤出法制备人工角膜的方法: 中国, CN01129695.X[P]. 2001-12-12
[7] 白华,黄一飞. Boston型人工角膜的临床并发症及其处理[J].眼科新进展,2013,33(9):886-888. BAI Hua, HUANG Yifei. Complications associated with Boston keratoprosthesis and relevant solutions[J]. Rec Adv Ophthalmol, 2013, 33(9):886-888.
[8] Akpek E K, Harissi-Dagher M, Petrarca R, et al. Outcomes of Boston keratoprosthesis in aniridia: a retrospective multicenter study[J]. Am J Ophthalmol, 2007, 144(2):227-231.
[9] Patel S, Takusagawa H, Shen L, et al. Long-term complications associated with glaucoma drainage devices and Boston keratoprosthesis[J]. Am J Ophthalmol, 2012, 154(1):207-208, author reply 208-209.
[10] Aldave A J, Sangwan V S, Basu S, et al. International results with the Boston type Ⅰ keratoprosthesis[J]. Ophthalmology, 2012, 119(8):1530-1538.
[11] Rudnisky C J, Belin M W, Todani A, et al. Boston type 1 keratoprosthesis study group. Risk factors for the development of retroprosthetic membranes with Boston keratoprosthesis type Ⅰ: multicenter study results[J]. Ophthalmology, 2012, 119(5):951-955.
[12] Sayegh R R, Ang L P, Foster C S, et al. The Boston keratoprosthesis in Stevens-Johnson syndrome[J]. Am J Ophthalmol, 2008, 145(3):438-444.
[13] Hicks C R, Hamilton S. Retroprosthetic membranes in Alpha-Cor patients: risk factors and prevention[J]. Cornea, 2005, 24(6):692-698.
[14] Hicks C R, Vijayasekaran S, Chirila T V, et al. Implantation of PHEMA keratoprostheses after alkali burns in rabbit eyes[J]. Cornea, 1998, 17(3):301-308.
[15] Lou X, Dalton P D, Chirila T V. Hydrophilic sponges based on 2-hydroxyethyl methacrylate. VII. Modulation of sponge characteristics by changes in reactivity and hydrophilicity of crosslinking agents[J]. J Mater Sci Mater Med, 2000,11(3): 319-325.
[16] Vijayasekaran S, Chirila T V, Robertson T A, et al. Constable IJ. Calci.cation of poly(2-hydroxyethylmethacrylate) hydrogel sponges implanted in the rabbit cornea: a 3-month study[J]. J Biomater Sci Polym Edn, 2000, 11(6):599-615.
[17] Haudek S B, Trial J, Xia Y, et al. Fc receptor engagement mediates differentiation of cardiac fi broblast precursor cells[J]. Proc Natl Acad Sci U S A, 2008, 105(29): 10179-10184.
[18] Zeisberg E M, Tarnavski O, Zeisberg M, et al. Endothelial-tomesenchymal transition contributes to cardiac fibrosis[J]. Nat Med, 2007,13(8): 952-961.
[19] Feldman D S, Elton T S, Sun B, et al. Mechanisms of disease: detrimental adrenergic signaling in acute decompensated heart failure[J]. Nat Clin Pract Cardiovasc Med, 2008, 5(4): 208-218.
[20] Bos R, Mougenot N, Findji L, et al. Inhibition of catecholamineinduced cardiac fibrosis by an aldosterone antagonist[J]. J Cardiovasc Pharmacol, 2005, 45(1): 8-13.
[21] Seeland U, Selejan S, Engelhardt S, et al. Interstitial remodeling in beta1-adrenergic receptor transgenic mice[J]. Basic Res Cardiol, 2007, 102(2): 183-193.
[22] 黎小间, 雷涛, 高建华. 苦味酸-天狼猩红偏振光法检测增生性瘢痕组织胶原[J]. 第一军医大学学报, 2002, 22(3):217. LI Xiaojian, LEI Tao, GAO Jianhua. Detection of collagens in hypertrophic scars by picrosirius polarization method[J]. J First Mil Med Univ, 2002, 22(3):217.
[23] 鲁元刚, 伍津津. 胶原在皮肤组织工程中的应用[J]. 重庆医学, 2003, 32(5): 609. LU Yuangang, WU Jinjin. Application of collagen in skin tissue engineering[J]. Chongqing Med J, 2003, 32(5):609.
[1] WANG Qun1, BAI Hua2, TIAN Lei2, WANG Li-qiang2, HUANG Yi-fei2. New soft one-piece PHEMA keratoprosthesis implanted into alkali burned rabbit corneas: an electron microscopy observation [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(3): 59-62.
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