JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (2): 40-45.doi: 10.6040/j.issn.1673-3770.0.2015.473

Previous Articles     Next Articles

Expressions and significance of TNF-α and IL-8 in serum and peripheral blood mononuclear cells from patients with sudden sensorineural hearing loss.

HENG Weiwei1,2, HOU Jie1, WEI Xianmei1, SHE Wandong1   

  1. 1. Department of Otolaryngology &Head and Neck Surgery, Affiliated Drum Tower Hospital, Medical College of Nanjing University, Nanjing 210008, Jiangsu, China;2. Department of Otolaryngology &Head and Neck Surgery, Nanjing Stomatological Hospital, Medical College of Nanjing University, Nanjing 210008, Jiangsu, China
  • Received:2015-11-09 Online:2016-04-16 Published:2016-04-16

PDF (PC)

525

Abstract: Objective To explore the expressions of tumor necrosis factor-α(TNF-α)and intedeukik-8(IL-8)in serum and peripheral blood mononuclear cells(PBMCs)from patients with sudden sensorineural hearing loss(SSNHL)and their clinical significance. Methods A total of 44 patients with SSNHL and 32 age-matched controls were enrolled in the study. The expressions of TNF-α and IL-8 in the serum and PBMCs were detected respectively. Results The expressions of TNF-α and IL-8 in the serum from patients with SSNHL were not much different from those in normal controls(P>0.05). However, they were significantly higher in PBMCs(P<0.05). Conclusion The elevated expressions of TNF-α and IL-8 in patients with SSNHL are closely related to the inner ear immune-mediation, indicating that inflammation may play an important role in the pathogenesis of the disease. The expressions of TNF-α and IL-8 may serve as novel diagnostic and therapeutic targets of SSNHL.

Key words: IL-8, Sudden sensorineural hearing loss, Inflammation, TNF-α, Immune-mediated, Serum, Peripheral blood mononuclear cell

CLC Number: 

  • R764.4
[1] 中华医学会耳鼻咽喉头颈外科学分会, 中华耳鼻咽喉头颈外科杂志编辑委员会. 突发性聋的诊断和治疗指南(2005年, 济南)[J]. 中华耳鼻咽喉头颈外科杂志, 2006, 41(8):325. Chinese Otorhinolaryngology Head and Neck Surgery Society of Chinese Medical Association, Editorial Board of Chinese Journal of Otorhinolaryngology Head and Neck Surgery. Guideline of diagnosis and treatment of sudden deafness(2005, Jinan)[J]. Chin J Otorhinolarynol Head Neck Surg, 2006, 41(8):325.
[2] Capaccio P, Pignataro L, Gaini L M, et al. Unbalanced oxidative status in idiopathic sudden sensorineural hearing loss[J]. Eur Arch Otorhinolaryngol, 2012, 269(2): 449-453.
[3] Kline M W, Frasch C E. Sudden-onset deafness: serodiagnosis of recent meningococcal infection[J]. Clin Pediatr(Phila), 1989, 28(11): 529-532.
[4] Yamasoba T, Kikuchi S, Higo R, et al. Sudden sensorineural hearing loss associated with slow blood flow of the vertebrobasilar system [J]. Ann Otol Rhinol Laryngol, 1993, 102(11):873-877.
[5] Simmons F B. The double-membrane break syndrome in sudden hearing loss [J]. Laryngoscope, 1979, 89(1): 59-66.
[6] Brookes G B. Immune complex-associated deafness: preliminary communication [J]. J R SocMed, 1985, 78(1): 47-55.
[7] Merchant S N, Adams J C, Nadol J B Jr. Pathologyand pathophysiology of idiopathic sudden sensorineural hearing loss [J]. Otol Neurotol, 2005, 26(2): 151-160.
[8] Merchant S N, Durand M L, Adams J C. Sudden deafness: is it viral? [J]. ORL J Otorhinolaryngol Relat Spec, 2008, 70(1): 52-62.
[9] Hajas A, Szodoray P, Barath S, et al. Sensorineural hearing loss in patients with mixed connective tissue disease: immunological markers and cytokine levels[J]. J Rheumatol, 2009, 36(9):1930-1936.
[10] Hiramatsu M, Teranishi M, Uchida Y, et al. Polymorphisms in genes involved in inflammatory pathways in patients with sudden sensorineural hearing loss [J]. J Neurogenet, 2012, 26(3-4):387-396.
[11] Okano T. Immune system of the inner ear as a novel therapeutic target for sensorineural hearing loss [J]. Front Pharmacol, 2014, 5(205):1-8.
[12] Schreiber B E, Agrup C, Haskard D O, et al. Sudden sensorineural hearing loss [J]. Lancet, 2010, 375(9721):1203-1211.
[13] Plontke S K, L(¨overo)wenheim H, Mertens J, et al. Randomized, double blind, placebo controlled trial on the safety and efficacy of continuous intratympanic dexamethasone delivered via a round window catheter for severe to profound sudden idiopathic sensorineural hearing loss after failure of systemic therapy [J]. Laryngoscope, 2009, 119(2):359-369.
[14] She W, Dai Y, Du X, et al. Hearing evaluation of intratympanic methyl prednisolone perfusion for refractory sudden sensorineural hearing loss [J]. Otolaryngol Head Neck Surg, 2010, 142(2):266-271.
[15] 孔维佳. 耳鼻咽喉科学[M]. 北京: 人民卫生出版社, 2007: 512.
[16] Schulmecht H F, Donovan E D. The pathology of idiopathic sudden sensorineural hearing loss[J]. Arch Otorhinolaryngology, 1986, 243(1):1-15.
[17] Juhn S K, Rybak L P, Pardo S. Nature of Blood-labyrinth Barrier in Experimental Conditions[J]. Ann Otol Rhinol Laryngol, 1981, 90(2):135-141.
[18] Garcia Berrocal J R, Ramirez-Camacho R. Immune response and immunopathology of the inner ear: an update[J]. Journal of Laryngology & Otology, 2000, 114(2):101-107.
[19] Kassner S S, Schöttler S, Bonaterra G A, et al. Proinflammatory Activation of Peripheral Blood Mononuclear Cells in Patients with Vestibular Neuritis [J]. Audiol Neurotol, 2011, 16(4):242-247.
[20] Kassner S S, Sch(¨overo)ttler S, Bonaterra G A, et al. Proinflammatory and proadhesive activation of lymphocytes and macrophages in sudden sensorineural hearing loss [J]. Audiol Neurotol, 2011, 16(4):254-262.
[21] Peter Libby, Paul M, Ridker. Inflammation and atherosclerosis [J]. Nature, 2002, 105(19): 1135-1143.
[22] 张胜权, 罗欣, 陈兵, 等. N-乙酰半胱氨酸体外下调 IL-8、IL-6及TNF-α在 PBMC中的表达[J]. 安徽医科大学学报, 2005, 40(1): 4-6. ZHANG Shengquan, LUO Xin, CHEN Bing, et al. N-acetylcysteine antagonizes the expression of IL-8, IL-6 and TNFα stimulated by PMA and A23187 in human peripheral blood mononuclear cells[J]. Acta Univ Med Anhui, 2005, 40(1):4-6.
[23] José R G, Rafael R C, José A G G, et al. Does the serological study for viral infection in autoimmune inner ear disease make sense?[J]. ORL, 2008, 70(1):16-20.
[24] Kara E, Cetik F, Tarkan O, et al. Modified intratympanic treatment for idiopathic sudden sensorineural hearing loss [J]. Eur Arch Otorhinolaryngol, 2010, 267(5):701-707.
[25] 严小玲, 毛敏, 翟锦明, 等. 鼓室内注射治疗难治性突发性耳聋52例[J]. 山东大学耳鼻喉眼学报, 2014, 28(5):23-26. YAN Xiaoling, MAO Min, ZHAI Jinming, et al. Intratympanic steroids to treat refractory sudden sensorineural hearing loss(52 cases report)[J]. J Otolaryngol Ophthalmol Shandong Univ, 2014, 28(5):23-26.
[26] 张彩霞, 刘阳云, 陈志喜, 等. 鼓室激素注射为初始治疗的突发性聋听力恢复的时间规律[J]. 山东大学耳鼻喉眼学报, 2015, 29(1):15-18. ZHANG Caixia, LIU Yangyun, CHEN Zhixi, et al. Time pattern of audiological recovery with intratympanic steroid injection as primary treatment for sudden sensorineural hearing loss[J]. J Otolaryngol Ophthalmol Shandong Univ, 2015, 29(1):15-18.
[27] Tom V B, Sofie V, Lien D, et al. Tumor Necrosis Factor Inhibits Glucocorticoid Receptor Function in Mice[J]. Journal of Biological Chemistry, 2011, 286(30):26555-26567.
[1] XU Ming, LUO Xinggu, TANG Hongbo, JIANG Qingshan. Clinical characteristics and prognostic factors for sudden idiopathic sensorineural hearing loss in children [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(5): 41-44.
[2] YANG Xue, LI Ying. Impact of dry eye on corneal endothelial cell density and morphology [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 72-76.
[3] PU Hongbo, DU Xiaodong. Allergen test results in 2 000 patients with allergic rhinitis in Wuxi District [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 105-107.
[4] REN Tao, TANG Yijun, WANG Meifang, LIU Yan, QIAN Xin.. Effect of obstructive sleep apnea-hypopnea syndrome on blood glucose, serum lipid, and C-reactive protein levels. [J]. J Otolaryngol Ophthalmol Shandong Univ, 2017, 31(6): 53-57.
[5] WU Yuanqing, XU Jinjing, DENG Yi. Experience in diagnosis and treatment of isolated inflammatory diseases of thesphenoid sinus. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 114-116.
[6] SHI Li, ZHAO Li, ZHANG Hongping. Long-term anti-inflammatory treatment of allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 9-12.
[7] . Clinical efficacy of Tiebifu Granule in the treatment of deafness with iron deficiency. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 75-79.
[8] . Serum lipid changes in obstructive sleep apnea hypopnea syndrome patients underwent airway surgery. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 27-30.
[9] . Efficacy of recombinant human epidermal growth factor in prevention of radioactive oral mucosa inflammation and acute radiation dermatitis for nasopharyngeal carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 95-99.
[10] JIN Chengxun, ZHAO Xue, LIU Yue, WEI Ning, JIN Chunshun. Report of 11 cases of Kimuras disease and review of literature. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 63-66.
[11] LIU Yangyun, ZHANG Caixia, JIANG Wen, CHEN Qiong, CHANG Enge, ZHAO Huaxiang, YAN Hui. Analysis of blood lipids in patients with sudden sensorineural hearing loss. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 1-4.
[12] LI Yanzhong. Obesity and obstructive sleep apnea. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 1-4.
[13] TAI Xuhui, YANG Xin, ZHU Baoyu, CAO Hong, CHU Yanling, SHAN Xiaodong, JIANG Yu. Analysis of allergen spectrum in 2 883 patients with allergic rhinitis in Liaoning area. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 39-42.
[14] LIN Li, YANG Xu. Analysis on efficacy of treatment of corneal epithelial dysfunction after cataract surgery. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 84-86.
[15] ZOU Juanjuan, WANG Yan, LI Yanzhong. Changes of serum myocardial enzymes and uric acid in patients with severe obstructive sleep apnea hypopnea syndrome complicated with hypertension [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(5): 1-5.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd