Abstract: Objective Autophagy was an important mechanism of protection and defense in organisms. The purpose of the study was observed the expressions of autophagy-related genes Atg3 and Ambra1, investigated the relevance between autophagy, chronic rhinosinusitis(CRS)without or with nasal polyps accompanied with asthma and discussed the probable mechanisms. Methods Collection the mucosa membrane tissue of inferior turbinate in patients with nasal septum deviation(n=24)as control group, research 3 groups included mucous in ostiomeatal complex with chronic rhinosinusitis without nasal polyps(CRSsNP)(n=21), chronic rhinosinusitis with nasal polyps(CRSwNP)(n=22)and chronic rhinosinusitis with nasal polyps accompanied with asthma(CRSwNP accompanied with asthma)(n=18). The expressions of Ambra1 and Atg3 in each group were detected by Immunohistochemistry. Relevance of the autophagy-related 山东大学耳鼻喉眼学报30卷2期 -张金陵,等.自噬相关基因Atg3、Ambra1与慢性单纯性鼻窦炎、鼻息肉及鼻息肉伴发哮喘的相关性研究 \=-genes among the groups was analyzed. Results (1)The expressions of autophagy-related genes Atg3 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were increased gradually(χ2=31.080, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.002), the control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P=0.002), CRSwNP and CRSwNP accompanied with asthma group(P=0.024)expression were statistically significant differences. However, there was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.304).(2)The expressions of autophagy-related genes Ambra1 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were all gradually increased(χ2=33.000, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.009), control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P<0.001), CRSwNP group and CRSwNP accompanied with asthma group(P=0.009)expression were statistically significant difference, There was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.205).(3)The expressions of Atg3 and Ambra1 were positively correlated in CRSsNP group(r=0.619, P=0.003), CRSwNP group(r=0.392, P=0.022)and CRSwNP accompanied with asthma group(r=0.552, P= 0.033)(P<0.05). However, there was no statistical correlation between Atg3 and Ambra1 in the contrast group(r=0.316, P=0.133)(P>0.05). Conclusion Autophagy is closely related with the CRS pathogenesis and may be involved in the occurrence of CRSwNP accompanied with asthma. Autophagy related genes Atg3 and Ambra1 may play a synergistic role in the development of CRS. Autophagy can be considered as a potential target for the diagnosis and therapeutic treatment in CRS.

Key words: Chronic rhinosinusitis, Ambra1, Autophagy, Atg3, Asthma