JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (1): 45-49.doi: 10.6040/j.issn.1673-3770.0.2017.027

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Effects of rosiglitazone on oxidative stress and cognitive function in mice exposed to intermittent hypoxia.

  

  1. 1. Department of Otolaryngology &Head and Neck Surgery;2. Sleep Center, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
  • Received:2017-01-17 Online:2017-02-16 Published:2017-02-16

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Abstract: Objective To study the effects of PPAR gamma agonist rosiglitazone on the oxidative stress and cognitive functionon in mice exposed to intermittent hypoxia. Methods Sixty healthy male ICR mice were randomly divided into: the normoxic control group(UC group, n=12), the intermittent hypoxia group(IH group, n=12), the low dose of rosiglitazone intervention group(1 mg/kg ROS+IH group, n=12), the middle dose of rosiglitazone intervention group(3 mg/kg ROS+IH group, n=12), and the high dose of rosiglitazone intervention group(9 mg/kg ROS+IH group, n=12). IH group and groups treated with rosiglitazone were exposed to intermittent hypoxia cabin with discontinuous gas, eight hours each day. After four weeks intervention, mice were divided into two parts. The MDA and SOD assay kits were used to measure MDA and SOD concentration of mice in part one and Morris water maze test was used to detect the learning and memory function of the remaining mice. Results Compared with the UC group, the MDA concentration increased(P<0.05)and the SOD concentration decreased(P<0.05)in the IH group; compared with IH group, MDA concentration of the groups treated with rosiglitazone decreased in a dose-dependent manner(P<0.05)and SOD concentration increased. Compared with group UC, the average escape latency became longer, the times of crossing the platform and the time percentage spent in the platform quadrant reduced in the IH group; compared with the IH group, average escape latency shortened, frequency of crossing the platform and the percentage of time spent in platform quadrant increased in the 9 mg/kg ROS+IH group(P<0.05). Conclusion PPAR gamma agonist-rosiglitazone can improve the oxidative stress damage and cognitive dysfunction induced by intermittent hypoxia in mice.

Key words: Oxidative stress, Sleep apnea, obstructive, Cognitive function, Intermittent hypoxia

CLC Number: 

  • R766.7
[1] 李涛平. 阻塞性睡眠呼吸暂停低通气综合征与多器官疾病的关系[J].中华肺部疾病杂志(电子版),2011,4(4):259-264. LI Taoping. Relationship between obstructive sleep apnea hypopnea syndrome and multiple organ disease[J]. Chin J Lung Dis(Elec Ed), 2011, 4(4):259-264.
[2] Yamauchi M, Kimura H. Oxidative stress in obstructive sleep apnea: putative pathways to the cardiovascular complications[J]. Antioxid Redox Signal, 2008,10(4):755-768.
[3] Hong S, Xin Y, HaiQin W, et al. The PPARγ agonist rosiglitazone prevents cognitive impairment by inhibiting astrocyte activation and oxidative stress following pilocarpine-induced status epilepticus[J]. Neurol Sci 2012,33(3):559-566.
[4] Chen T, Jin X, Crawford BH, et al. Cardioprotection from oxidative stress in the newborn heart by activation of PPARγ is mediated by catalase[J]. Free Radic Biol Med,2012,53(2):208-215.
[5] 王彤宇, 吴广才, 孙冬霞, 等. 吡格列酮治疗血管性认知功能障碍的临床观察[J].中华老年心脑血管病杂志,2013,15(11):1174-1177. WANG Tongyu, WU Guangcai, SUN Dongxia, et al. Application of pioglitazone in treatment of vascular cognitive impairment[J]. Chin J Geria Heart Brain Vessel Dis, 2013, 15(11):1174-1177.
[6] Vorhees CV, Williams MT. Morris water maze: procedures for assessing spatial and related forms of learning and memory[J]. Nat Protoc, 2006, 1(2):848-858.
[7] 王青云, 陈锐, 曹忠胜, 等. 同期多平面手术对中青年中-重度OSAHS患者血清氧化应激水平和认知功能的影响[J].临床耳鼻咽喉头颈外科杂志,2015,29(24): 2139-2142. WANG Qingyun, CHEN Rui, CAO Zhongsheng, et al. Effects of simultaneous multi-level surgery intervention on the changes of the oxidative stress and cognitive function in young and middle-aged patients with Moderate-severe obstructive sleep apnea hypopnea syndrome[J]. J Clin Otorhinolaryngol Head Neck Surg, 2015, 29(24): 2139-2142.
[8] Tauman R, Lavie L, Greenfeld M, et al. Oxidative stress in children with obstructive sleep apnea syndrome[J]. J Clin Sleep Med, 2014,10(6):677-681.
[9] 李亚男, 陈锐, 刘辉玉, 等. 间歇低氧小鼠血清氧化应激指标的变化及N-乙酰半胱氨酸的治疗作用[J].江苏医药,2011,37(4):387-389. LI Yanan, CHEN Rui, LIU Huiyu, et al. The changes of serum oxidative stress indexes of mice exposed to intermittent hypoxia and the therapeutical effect of N-acetylcysteine[J]. Jiangsu Med J, 2011, 37(4):387-389.
[10] 琚静美, 陈锐, 王婧, 等. 慢性间歇低氧诱导小鼠学习记忆障碍的实验研究[J]. 中华肺部疾病杂志(电子版),2014,7(6):10-13. JU Jingmei, CHEN Rui, WANG Jing, et al. Experimental study on the impairment of spatial learning and memory in the mice exposed to chronic intermittent hypoxia[J]. Chin J Lung Dis(Elec Ed), 2014, 7(6):10-13.
[11] Yu X, Shao XG, Sun H, et al. Activation of cerebral peroxisome proliferator-activated receptors gamma exerts neuroprotection by inhibiting oxidative stress following pilocarpine-induced status epilepticus[J]. Brain Res, 2008, 1200:146-158.
[12] Murphy GJ, Holder JC. PPAR-gamma agonists: therapeutic role in diabetes, inflammation and cancer[J]. Trends Pharmacol Sci, 2000, 21(12):469-474.
[13] Collino M, Aragno M, Mastrocol R, et al. Modulation of the oxidative stress and inflammatory response by PPAR-gamma agonists in the hippocampus of rats exposed to cerebral ischemia/reperfusion[J]. Eur J Pharmacol, 2006, 530(1-2):70-80.
[14] 罗颜, 曹忠胜, 朱政文, 等. 15D-PGJ2对小鼠耳蜗基底膜螺旋神经节氧化损伤的防护作用[J].中华耳科学杂志,2015,13(1):71-75. LUO Yan, CAO Zhongsheng, ZHU Zhengwen, et al. Protective effect by 15D-PGJ2 against oxidative damage to mouse cochlear spiral ganglion[J]. Chin J Otol, 2015, 13(1):71-75.
[15] 王宁, 韩晶, 徐艳炜, 等. 过氧化氢损伤原代皮质神经元中PPARγ的改变[J].中华医学杂志,2015,95(21):1686-1690. WANG Ning, HAN Jing, XU Yanwei, et al. Hydrogen peroxide-induced changes of PPARγ in primary cultured cortical neurons[J]. Nat Med J Chin, 2015, 95(21):1686-1690.
[16] 姜云苓, 李广群, 沈洁, 等. 罗格列酮对慢性阻塞性肺疾病大鼠模型肺组织氧化应激的影响[J].中国老年学杂志,2015,35(7):1893-1894. JIANG Yunling, LI Guangqun, SHEN Jie, et al. Effects of rosiglitazone on oxidative stress in lung tissue of rats with chronic obstructive pulmonary disease[J]. Chin J Gerontol, 2015, 35(7):1893-1894.
[18] Hwang J, Kleinhenz DJ, Lassegue B, et al. Peroxisome proliferator-activated receptor-gamma ligands regulate endothelial membrane superoxide production[J]. Am J Physiol Cell Physiol, 2005, 288(4):C899-905.
[19] Jennings JS, Gerber AM, Vallano ML. Pharmacological strategies for neuroprotection in traumatic brain injury[J]. Mini Rev Med Chem, 2008, 8(7):689-701.
[20] 邓永兵, 唐文渊. PPARγ的神经保护作用研究进展[J].中华神经外科疾病研究杂志,2010,9(1):92-94. DENG Yongbing, TANG Wenyuan. Research progress on neuroprotection of PPAR-gamma[J]. Chin J Neurosurg Dis Res, 2010, 9(1):92-94.
[21] Searcy JL, Phelps JT, Panncani T, et al. Long-term pioglitazone treatment improves learning and attenuates pathological markers in a mouse model of Alzheimers disease[J]. J Alzheimers Dis, 2012, 30(4):943-961.
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