JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (5): 72-78.doi: 10.6040/j.issn.1673-3770.0.2017.297

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Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells.

ZHANG Caixia1,2, LIU Yangyun1, JIANG Wen1, LIU Gengxun1, CAO Hang1, CHEN Qiong1, ZHANG Jishuai1   

  1. Department of Otolaryngology, 1. No.163 Hospital of PLA /The Second Affiliated Hospital of Hunan Normal University Medical College, Changsha 410003, Hunan, China;2. Liuzhou Workers Hospital / Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi, China
  • Received:2017-07-07 Online:2017-10-16 Published:2017-10-16

Abstract: Objective To investigate whether exogenous nitric oxide(NO)could increase chemosensitization to cisplatin(DDP)in CNE2 cells, and to provide an experimental and theoretical basis for improving the effectiveness of chemotherapy for nasopharyngeal carcinoma. Methods CNE-2 cells were treated with various concentrations of sodium nitroprusside(SNP), DDP, and both chemicals. The nitrate reductase method was used to detect the concentration of NO. Morphological changes of cells were observed using an inverted phase contrast microscope and CCK8 assays were used to examine the viability of cells. Flow cytometry was applied to detect apoptosis in NPC cells. Results (1)The concentration of NO was positively correlated with the concentration of SNP, and this correlation was statistically significant(r=0.968, P<0.05).(2)Compared with the CNE-2 cells in the SNP group, DDP group, and control group, the CNE-2 cells in the DDP+SNP group showed more extensive morphological changes.(3)The inhibitory effects of SNP+DDP were significantly greater than those of SNP or DDP alone(P<0.05).(4)Compared with the groups treated with SNP or DDP individually, flow cytometry showed that the apoptosis rate of CNE-2 cells was significantly higher in the group treated with both SNP and DDP(P<0.05). Conclusion Exogenous NO can inhibit CNE-2 proliferation, and the inhibitory effect was positively correlated with the concentration of NO. The proper concentration of exogenous NO can significantly enhance the chemosensitivity of CNE-2 cells to DDP, without substantial toxicity.

Key words: Nasopharyngeal carcinoma, Sodium nitroprusside, Cis-platinum, Apoptosis, Chemosensitization, Nitric oxide

CLC Number: 

  • R739.62
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