JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2018, Vol. 32 ›› Issue (2): 84-87.doi: 10.6040/j.issn.1673-3770.0.2017.323

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A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells

ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui   

  1. Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xian Jiaotong University, Xian 710004, Shaanxi, China
  • Received:2017-07-28 Online:2018-03-20 Published:2018-03-20

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Abstract: Objective The aim of this study was to explore the anti-tumor potential of a polysaccharide isolated from Hedyotis diffusa(HDP)in Hep-2 human larynx squamous carcinoma cells. Methods Cell cycle and apoptosis were analyzed by detecting changes in protein expression by flow cytometry. Apoptosis was further characterized by detecting relevant proteins by western blotting. Results HDP suppressed the proliferation of Hep-2 cells in a time- and dose-dependent manner. Cell cycle analysis revealed that exposure to HDP(400 μg/mL)caused G0/G1 cell cycle arrest in Hep-2 cells. Moreover, flow cytometry revealed that Hep-2 cells treated with HDP for 24 h underwent apoptosis to a significantly greater extent than untreated control cells. HDP treatment promoted the expression of caspase-3, caspase-8, and caspase-9, and reduced the expression of Bcl-2. HDP also inhibited cell migration and suppressed the expression of MMP-2 and uPA. Conclusion HDP possesses suppressive effects against aggressive phenotypes of human larynx squamous carcinoma cells and may have therapeutic potential for this particular malignancy.

Key words: Hedyotis diffusa, Polysaccharide, Hep-2 Cells, Apoptosis, Migration

CLC Number: 

  • R739.6
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