JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2018, Vol. 32 ›› Issue (3): 47-53.doi: 10.6040/j.issn.1673-3770.0.2018.068

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BRF2 in the pathogenesis of chronic rhinosinusitis with nasal polyps

GAN Bin1, ZHANG Yongju1, XU Anting2   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2018-01-27 Online:2018-05-20 Published:2018-05-20

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Abstract: Objective To study the expression and significance of BRF2 in chronic rhinosinusitis with nasal polyps. Methods 37 surgical specimens of chronic rhinosinusitis with nasal polyps(CRSwNP)and 18 surgical specimens of simple deviation of nasal septum were collected from 2016 to 2017 in the Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University. Immunofluorescence staining was used to detect the expression and localization of BRF2 in chronic rhinosinusitis with nasal polyps. After cultured human nasal mucosa epithelial cells were stimulated with Llipopolysaccharides(LPS)in vitro, the expression and localization of BRF2 in cells were detected by immunofluorescence staining. Western blotting and quantitative real-time PCR were used to detect the expression of BRF2 and the endoplasmic reticulum stress-related protein GRP78 in cells. Results The expression of BRF2 in chronic rhinosinusitis with nasal polyps was up-regulated in whole cells and was particularly increased in the cytoplasm fraction. In vitro, LPS stimulation can induce the up-regulation of expression of BRF2 to be up-regulated in nasal mucosal epithelial cells and increase the distribution of BRF2 in the cytoplasm, and tThe expression of endoplasmic reticulum stress-related protein GRP78 was also increased. Conclusion BRF2 may mediate endoplasmic reticulum stress, which is involved in the pathogenesis of chronic rhinosinusitis with nasal polyps.

Key words: Human nasal epithelial cells, BRF2, GRP78, Nasal polyps, Rhinosinusitis, chronic

CLC Number: 

  • R765.4+1
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