山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (5): 38-41.doi: 10.6040/j.issn.1673-3770.0.2015.448

• 睡眠呼吸障碍性疾病 • 上一篇    下一篇

间歇低氧模型大鼠肝脏组织中IGF-1、IGFBP-3的表达及分析

高天喜,夏翠,闫静,侯瑾,康全清,王正辉,任晓勇   

  1. 西安交通大学第二附属医院耳鼻咽喉头颈外科病院, 陕西 西安 710004
  • 收稿日期:2015-10-27 出版日期:2016-10-20 发布日期:2016-10-20
  • 通讯作者: 侯瑾. E-mail:13319251982@126.com E-mail:tanghede@126.co
  • 作者简介:高天喜. E-mail:tanghede@126.co
  • 基金资助:
    国家自然科学基金(30772404)

Analysis of IGF-1 and IGFBP-3 level in liver tissue of rat with intermittent hypoxia.

GAO Tianxi, XIA Cui, YAN Jing, HOU Jin, KANG Quanqing, WANG Zhenghui, REN Xiaoyong   

  1. Department of Otolaryngology &Head and Neck Surgery, Second Affiliated Hospital of Xian Jiaotong University, Xian 710004, Shaanxi, China
  • Received:2015-10-27 Online:2016-10-20 Published:2016-10-20

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摘要： 目的 检测阻塞性睡眠呼吸暂停低通气综合征(OSAHS)间歇性低氧模型大鼠肝脏组织中IGF-I及IGFBP-3水平的变化,探讨间歇低氧对肝脏的影响,为研究儿童OSAHS的病理生理改变提供客观依据。 方法 24只雌性20日龄SD大鼠随机分为对照组、轻度低氧组、重度低氧组,轻度低氧组、重度低氧组大鼠饲养于间歇性缺氧密闭箱内8 h/d。35 d后处死取材,免疫组织化学法检测肝脏IGF-1、IGFBP-3。 结果 对照组、轻度低氧组、重度低氧组肝组织IGF-1的阳性表达分别为2/8、3/8和6/8,IGFBP-3的阳性表达分别为3/8、4/8和7/8,有逐渐升高趋势。对照组与轻度低氧组肝组织IGF-1、IGFBP-3的表达差异无统计学意义。对照组与重度低氧组肝组织IGF-1、IGFBP-3的表达差异无统计学意义。Spearman秩相关检验表明,重度低氧组中IGF-1与IGFBP-3的高表达呈正相关(r=0.746, P<0.05)。 结论 间歇性低氧模型中大鼠肝细胞IGF-1与IGFBP-3含量受生长激素(GH)-IGF1-IGFBP3轴功能紊乱的影响不大,重度低氧组大鼠肝细胞IGF-1与IGFBP-3含量与对照组及轻度低氧组比较无显著差异。

关键词: 胰岛素样生长因子-l, 肝脏, 胰岛素样生长因子结合蛋白-3, 阻塞性睡眠呼吸暂停低通气综合征

Abstract: Objective To detect the insulin-like growth factor 1(IGF-1)and insulin-like growth factors binding protein 3(IGFBP-3)levels in liver tissue of rat with intermittent hypoxia, and to investigate theeffect of intermittent hypoxia on liver, in order to provide pathophysiologic evidence of obstructive sleep apnea-hypopnea syndrome(OSAHS)in children. Methods A total of 20 day-old Sprague-Dawley female rats were randomly divided into 3 groups: mild hypoxia group, severe hypoxia group and control group, the first two groups were raised in sealed hypoxia box 8-hour per day and were sacrificed 35 days after, The level of IGF-1 and IGFBP-3 of liver tissure was detected by immunohistochemical method. Results The expression of IGF-1 and IGFBP-3 of liver in control group, mild hypoxia group and severe hypoxia group increased gradually. The positiveexpression rate of IGF-1 were 2/8, 3/8 and 6/8 in control group, low oxygen group and severe hypoxia group, respectively. The positiveexpression rate of IGFBP-3 were 3/8、4/8 and 7/8 in there group respectively. There was no significant difference in level of IGF-1 and IGFBP-3 between the control group and the mild hypoxia(χ=20.157, P=0.315), and between the control group and thesevere hypoxia(χ=20.066, P=0.132). Spearman-test showed a positive correlation between level of IGF-1 and IGFBP-3 in severe hypoxia group(r=0.746, P<0.05). Conclusion GH-IGF1-IGFBP3axisdysfunctionhas little effect on IGF-1and IGFBP-3 in liver tissue of rate withintermittent hypoxia.There was no significant difference in level of IGF-1 and IGFBP-3 among three groups.

Key words: Insulin-likegrowth factorl, obstructive sleep apnea-hypopnea syndrome, insulin-likegrowth factor binding protein-3, liver

中图分类号: 

  • R766.7
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