Abstract:

Objective  To explore the methods to establish a tumor bank for head and neck carcinoma by collecting and preserving the tumor tissue specimens with an information-based database. Methods   The tumor tissues, tumor-adjacent tissues, normal tissues were collected from surgery or biopsy, as well as peripheral blood samples, including serum, plasma, lymphocyte or stem cells, etc, from head and neck carcinoma patients and healthy subjects.  All the specimens were stored in liquid nitrogen or -80℃ refrigerator. Meanwhile, an information-based database was established.  Results   Specimens from 220 cases were collected, including 120 cases of carcinoma (tumor and tumor-adjacent tissues) and 100 cases of tissues without tumor. Samples of peripheral blood from 476 cases were collected, including 316 cases of carcinoma and 160 healthy subjects. Conclusion   The tissue bank of head and neck carcinoma can be established large-scalely and intensively,  which  can greatly enhance the efficiency of specimen usage.

Key words: Head and neck carcinoma, Tissues bank, Establishment