miR-204通过内质网应激抑制视网膜母细胞瘤生长作用及机制的研究

doi:10.6040/j.issn.1673-3770.0.2018.132

Abstract

Abstract: Objective To evaluate miR-204 expression in human retinoblastoma and to investigate the effect of latter's growth and molecular mechanism. Methods RT-PCR was performed to detect the expression of miR-204 in retinoblastoma(Y79)and the normal retinal(ARPE-19)cell lines. The mimic(s)and inhibitor(s)of miR-204 were transiently transfected into Y79 cells. CCK-8 and flow cytometry aided the detection of the survival and apoptosis of Y79 cells. The expression level of GRP78 protein was determined by Western blot. Results The expression of miR-204 in Y79 cells was significantly lower than that in ARPE-19. It was observed that the over-expressed miR-204 in Y79 cells could significantly inhibit the proliferation of retinoblastoma. Moreover, it promoted apoptosis, increased GRP78 protein's expression, and could activate the endoplasmic reticulum stress pathway. Conclusion miR-204 expression in retinoblastoma cells was significantly lower than that in the normal retinal cells. In addition, miR-204 might induce retinoblastoma cell(s)apoptosis by activating the endoplasmic reticulum stress pathway.

Key words: miR-204, Retinoblastoma, Endoplasmic reticulum stress

CLC Number:

R774.1

SU Jie, MA Chunmei, ZHAO Lili, LIU Yan, SHAO Hongchao. Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 105-108.

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Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress

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