JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2011, Vol. 25 ›› Issue (3): 5-8.

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Expression and clinical significance of TERT and PCNA in the tissue and cell lines of laryngeal squamaous cell carcinoma

CHEN Jin-hui, TAO Ze-zhang   

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2010-10-07 Revised:2011-01-22 Online:2011-06-16 Published:2011-06-16

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Abstract:

Objective     To investigate expression of TERT(telomerase reverse transcriptase) and PCNA(proliferating cell nuclear antigen) in the tissue and cell lines of laryngeal squamaous cell carcinoma, and to elucidate the clinical pathological significance. Methods      A continuous mutiple-spectrums scan was applied to show expression of TERT and PCNA in tissue array. TERT and PCNA mRNA expression was measured in Hep-2 cells by RT-PCR test after siRNA inhibition. Results       Expressions of TERT and PCNA were significantly different in benign and malignant head and neck cancers. Among the malignant, expression of TERT and PCNA were all higher in grade Ⅲ than grade Ⅰ-Ⅱ . Also, both expressions were moderately interrelated(r=0.577, P<0.05). TERT mRNA level was decreased by 80% after siRNA inhibition, while the PCNA mRNA level was decreased by 70%. Conclusion      TERT and PCNA are related to the development of laryngeal squamaous cell carcinoma. They are synergetic. It is possible that PCNA can exert an effect on TERT to modulate the proliferation of cancer cells.

Key words: TERT; PCNA; Quantum dot; RNA interference

CLC Number: 

  • R739.6
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