Tespa1在慢性鼻窦炎伴鼻息肉中的表达及作用研究

doi:10.6040/j.issn.1673-3770.0.2024.108

Abstract

Abstract: Objective To determine the expression and expression differences of thymocyte-expressed, positive selection associated 1(Tespa1)in nasal polyps and nasal mucosa and to investigate the role of Tespa1 in CRSwNP and its relationship with mast cells. Methods Twenty-nine patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and 20 patients with chronic rhinosinusitis without nasal polyps(CRSsNP)were included in the study, 18 cases of controls. Quantitative real-time PCR(qPCR), immunohistochemistry(IHC), enzyme-linked immunosorbent assay(ELISA), and hematoxylin-eosin(H&E)staining were used to detect the expression and distribution of Tespa1 in nasal mucosa and nasal polyps, and its correlation with mast cells, IgE, IL-4, and IL-13 was analyzed. Results The qPCR results showed that the expression level of Tespa1 mRNA in CRSwNP was significantly lower than that in both control and CRSsNP groups(Z=2.42, P=0.046; Z=-3.38, P=0.002). The IHC results showed that Tespa1 was lowly expressed in the epithelium and mesenchyme of nasal polyps compared with the control group, and the expression of tryptase-positive mast cells was upregulated in nasal polyps(t=10.14, P<0.001). The number of Tespa1-positive cells in nasal polyps was negatively correlated with the number of trypsin-positive mast cells(r=-0.710, P=0.006).The ELISA results showed that the tissue Tespa1 level was much higher than its level in serum, and the tissue Tespa1 level was negatively correlated with the levels of IgE, IL-4, and IL-13(r=-0.682, P=0.002; r=-0.682, P=0.002; r=-0.691, P=0.002; r=-0.539, P=0.021). Conclusions Tespa1 is low expressed in nasal polyp tissues of CRSwNP patients, negatively correlated with the expression levels of IgE, IL-4 and IL-13, and may act through mast cells. This study provides a new perspective for understanding the pathomechanism of CRSwNP and may provide new molecular targets for future diagnosis and treatment.

Key words: Chronic rhinosinusitis with nasal polyps, Thymocyte expressed, positive selection associated 1, Mast cell

CLC Number:

R765.4

ZHANG Guangling, CHEN Xingxue, WU Tianyi, SUN Zhanwei, WANG Weiwei, LI Shichao,WANG Guangke. The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 35-42.

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The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps

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