JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (1): 44-48.doi: 10.6040/j.issn.1673-3770.0.2014.272

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Allograft rejection after high risk corneal transplantation with Foxp3 Gene-transferred effective T cells

ZHAO Jun1 2, LIU Jing1, WANG Da-jiang3, CHEN Guo-ling1, WANG Qi2, ZHANG Han1   

  1. 1. Liaohe Oilfield General Hospital, Panjin 124010, Liaoning, China;
    2. The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Chinese PLA General Hospital, Beijing 100853, China
  • Received:2014-08-17 Online:2015-02-16 Published:2015-02-16

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Abstract: Objective To observe the effects of Foxp3 gene-transfected Teff lymphocytes against the high-risk corneal transplantation rejection. Methods The Foxp3 gene-transfected Teff lymphocytes and the Teff lymphocytes were cultured together to observe the inhibitory effects on the Teff lymphocytes. Corneal neovascularization was induced by corneal suture in Balb/c mice and after one week, penetrating corneal transplantations were performed while Balb/c mice as receptors and C57/BL6 mice as donors. One day before the surgery, injection of Foxp 3 gene-transfected Teff lymphocytes were performed via tail vein of Balb/c mice. After the surgery, the state of corneal transplantation was monitored on a day to day basis and tail vein blood was sampled once every two weeks to check the lymphocyte types. Results The in vitro experiments showed strong inhibitory effects of Foxp3 gene-transfected Teff lymphocytes on the Teff lymphocytes. To the high-risk corneal transplantation mice, the Foxp3 gene-transfected Teff lymphocytes could moderately extend the survival time of the grafts (19.71±4.19d) while the survival time of the corneal grafts in the Treg group was 19.28±4.46d (P>0.05). On the contrary, the comparison between the Foxp3 gene-transfected Teff lymphocytes and the blank group (15.57± 2.22d) demonstrated some significance (P<0.05). Flow cytology indicated that the Foxp3 gene-transfected lymphocytes could delay the proliferation of CD4+T and CD8+T lymphocytes.Conclusion The Foxp3 gene-transfected Teff lymphocytes could prevent transplant rejection.

Key words: Teff, Treg, High risk, Foxp3 gene, Penetrate cornea transplant, Allograft rejection

CLC Number: 

  • R779.6
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