JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (4): 76-79.doi: 10.6040/j.issn.1673-3770.0.2016.477

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The effect of transforming growth factor-β1 on epithelial-mesenchymal transition in human lens epithelial cells.

WANG Hui1, WEN Yan1, YAN Li1, YU Xiaoming1, NING Hong   

  1. 1. Department of Ophthalmology, Shandong Provincial Traffic Hospital, Jinan 250031, Shandong, China;2. Department of Ophthalmology, The Fourth Hospital of China Medical University, Shenyang 110000, Liaoning, China
  • Received:2016-11-17 Online:2017-08-16 Published:2017-08-16

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Abstract: Objective To investigate the effect of transforming growth factor(TGF)-β1 on the epithelial-mesenchymal transition(EMT)in human lens epithelial cells(HLECs). Methods HLECs were cultured separately, according their treatment group. For this purpose, cells were divided into the TGF-β1 treatment group, siRNA-TGF-β1 group, and the control group. After culturing cells for 24 h in their respective treatment groups, cell morphologies were observed. Cell migration in each treatment group was detected using the transwell migration assay. The expression levels of TGF-β1, α-SMA, E-cadherin, and vimentin genes were detected using real-time polymerase chain reaction. The expression levels of PI3K, Akt, and p-Akt proteins were detected using Western blotting. Results In the TGF-β1 treatment group, 31.2±4.7 HLECs were found to have transformed. This number was significantly higher than that in the siRNA-TGF-β1(14.6±5.3)and control groups(3.9±1.4). Specifically, the number of cells transformed in the siRNA-TGF-β1 group was higher than that in the control group. Expression levels of TGF-β1 mRNA, α-SMA mRNA, and vimentin mRNA were the highest in the TGF-β1 treatment group, followed by lower levels in the siRNA-TGF-β1 group, and the lowest levels in the control group. In contrast, the expression levels of E-cadherin mRNA were highest in the siRNA-TGF-β1 group, followed by lower levels in the control group and the lowest levels in the TGF-β1 treatment group. These differences were statistically significant(P<0.05). The expression levels of PI3K and p-Akt proteins were the highest in the cells of the TGF-β1 treatment group, followed by lower levels in the control group. These levels were lowest in the siRNA-TGF-β1 group. The relative expression levels of Akt proteins were the highest in the siRNA-TGF-β1 group, followed by lower levels in the control group and the lowest levels in the TGF-β1 treatment group; these differences were statistically significant(P<0.05). Conclusion TGF-β1 could induce HLECs towards EMT, and thus promote HLEC migration. This mechanism could be related to the activation of PI3K/Akt signaling pathway.

Key words: TGF-β1, human lens epithelial cells, Epithelial-mesenchymal transition, PI3K/Akt signaling pathway

CLC Number: 

  • R776.1
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