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Expression of the heat shock protein 27 in rat cochlea induced by sodium salicylate injection by the FQ-PCR technique

FAN Qi-jun1, HUANG Zhi-wu2, MEI Ling2, XIAO Bo-kui2   

  1. 1. Department of Otolaryngology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang, China; 2. Department of Otolaryngology & Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China
  • Received:2008-02-19 Revised:2008-04-23 Online:2008-06-16 Published:2008-06-16
  • Contact: HUANG Zhi-wu

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Abstract: To investigate the gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection and to explore its role in sodium salicylate ototoxity. MethodsThe HSP27 gene expression in specimens was determined by using real time RT-PCR with SYBR GREEN I, and β-actin served as the internalcontrol. ResultsThe gene expression of HSP27 in the cochlea of rats induced by sodium salicylate injection was significantly increased compared with normal rats(P＜0.01). ConclusionQuantitative PCR with SYBR GREEN I fluorescence is a reliable method to determine the gene expression of rarely available tissues. Sodium salicylate has an effect on inducing the gene expression of HSP27 in the cochlea of rats.

Key words: Cochlea, Gene, Heat shock protein 27, Sodium salicylate, Rat

CLC Number: 

  • R764
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