JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

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Polymorphism of mitochondrial DNA at the D-310 region in laryngeal squamous cancer cells

HAN Yue-chen, FAN Zhao-min, XU Wei, LI Jian-feng, L〖AKU¨] Zheng-hua, WANG Hai-bo

  

  1. (Department of Otolaryngology & Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-16 Published:2008-08-16
  • Contact: WANG Hai-bo

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Abstract:

Objective〓〖WTBZ〗To investigate the polymorphism of the D310 region of somatic mitochondrial DNA (mtDNA) in the human primary laryngeal squamous cell cancer. 〖WTHZ〗Methods〓〖WTBZ〗Eleven cases of laryngeal squamous cell cancer were collected from in-patients who were confirmed by pathologic studies. The total DNA was amplified to identify the polymorphism of the D-310 region by the PCR technique, polyacrylamide gel electrophoresis and sequencing. 〖WTHZ〗Results〓〖WTBZ〗Changes in length of short C base-repetitive sequencing were found in the conserved D310 region in 8 cases (72.73%). Most of the mutations (8/11) were homoplasmic mutations, and the others (3/11) were hytroplasmic mutations. Of the 6 kinds of mutation changes, insertion of one cytosine at the D-310 region was the most common, ranked about 50%. 〖WTHZ〗Conclusions〓〖WTBZ〗The D310 region located in the D-Loop region could be a mutational hotspot in laryngeal squamous cell cancer.

Key words: Mutation, Laryngeal squamous cell cancer,

Mitochondrial DNA, D-310 Region

CLC Number: 

  • R739.65
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