JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (3): 16-19.doi: 10.6040/j.issn.1673-3770.0.2015.087

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Cytocompatibility research of schwann cells and neural stem cells co-seeded on electron spinning chitosan-PLGA

MEN Yongzhi, CHENG Yusheng, LI Yu, DONG Pin, YU Ziwei   

  1. Department of Otolaryngology & Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai 200080, China
  • Received:2015-02-10 Revised:2015-04-10 Published:2015-06-16

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Abstract: Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells (SCs)and neural stem cells(NSCs) as well as cells adhesion and migration on the arranged nanofibers inside the lumen. Methods Experiment was divided into three groups: the SCs group, the NSCs group and the SCs-NSCs co-culture group. Cells in these groups were co-seeded with Chitosan-PLGA. The biocompatibility and absorbability of SCs and NSCs were detected by MTT assays. Scanning electron microscopy(SEM) was used to detect cells adhesion and migration on the axially arranged nanofibers inside the lumen. Results Cell survival rate of the co-culture group was the highest, the higher in the SCs group, and the lowest in the NSCs group. SCs and NSCs had good compatibility and adhesion with Chitosan-PLGA, and the axial nanofibers in the lumen guided the growth of neurites in a particular direction. Conclusion Co-culture of SCs and NSCs can facilitate cells compatibility and adhesion with 3D Chitosan-PLGA, and the axial arranged nanofibers within the lumen has the effect of directional guide cell growth and migration.

Key words: Electron spinning, Chitosan-PLGA, Schwann cells, Neural stem cells, Directional growth

CLC Number: 

  • R745.1
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