Abstract:

Objective   To investigate the changes of Tregs and lymphocyte subpopulations in the peripheral blood of the patients with SOM (secretory otitis media),  and explore the influence of immunological status on the development of SOM.  Methods   Peripheral blood samples of thirtynine patients and thirty healthy donors were prepared. The percent of CD4+CD25+、CD3+、CD4+、CD8+、NK、B lymphocyte and the ratio of CD4+/CD8+ were  analyzed by tricolour flow cytometry. Results   The percentage of CD3+CD8+、NK in the patients group was lower than that of the control group(P<0.05)；The percentage of  CD4+/CD8+、B lymphocyte was  higher than that of the control group. The percentage of Tregs in the patient  and  control group were (12.82±4.56)% and (6.695±1.09)%, respectively(P<0.05). There were no significant differences in the number of  CD3+、CD3+CD4+、CD3+CD8+T lymphocyte and the percentage of  CD3+、CD4+ T lymphocyte. Conclusion   The ratio of CD4+/CD8+ in the SOM patients is significantly lower than that of the healthy and the ratio of Tregs is higher, which implies that immune imbalance existes in the acute secretory otitis media patients.

Key words:  Secretory otitis media； Flow eytometry； Lymphocyte