Abstract:

Objective To investigate the role of human papillomavirus (HPV) in development of laryngeal squamous cell carcinoma by detecting expression of HPV DNA and its different subtypes in easter of the Shandong province. Methods Fifty-seven patients with laryngeal carcinoma treated in our department were enrolled， and， in the same period， eight patients with papilloma of the larynx served as the control group. HPV subtypes were detected in both groups with a DNA chip technology combining polymerase chain reaction (PCR) with DNA reverse point hybrid. Results Only 4(7.02%)were positive in 57 cases of laryngeal carcinoma specimens， and the positive rate was lower than that in laryngeal papilloma(6/8， 75%)(χ2=24.906，P＜0.01). Respectively comparing the expression of highrisk HPV16 and low-risk HPV43 subtypes between the both groups， we did not find significant difference (χ2=5.611,0.143，P＞0.05)； expressions of lowrisk HPV11 and HPV6 in laryngeal papilloma were higher than those in laryngeal carcinoma (χ2= 8.611,14.702，P<0.05). Conclusion The rate of HPV infection is low among laryngeal carcinoma patients in easter of the Shandong province. Low-risk HPV6 and HPV11 may be closely related with the occurrence of laryngeal papilloma.

Key words: Laryngeal neoplasms； Carcinoma， squamous cell； Human papillomavirus； Polymerase chain reaction