JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2014, Vol. 28 ›› Issue (4): 81-84.doi: 10.6040/j.issn.1673-3770.0.2013.172

Previous Articles     Next Articles

Expression of survivin in animal model of posterior capsule opacification

LIU Shu-jun1, DAI Xiu-yu1, LIU Shao-yi1, WANG Wen-ting2, LI Yuan-bin1   

  1. 1. Department of Ophthalmology, Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264000, Shandong, China;
    2. Central Laboratory, Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264000, Shandong, China
  • Received:2013-06-13 Online:2014-08-16 Published:2014-08-16

PDF (PC)

260

Abstract: Objective To establish animal model of posterior capsule opacification (PCO) in New Zealand white rabbits and detect survivin expression in PCO tissue. Methods Thirty adult New Zealand white rabbits were randomly divided into control group and experimental group. The 5 rabbits in control group were executed. Ultrasonic phacoemulsification was performed in the 25 experimental rabbits' eyes. Their eyes were examined by slit lamp microscope to observe the development of PCO immediately and 3, 7, 14 and 28 days after surgery, and then they were executed. The posterior capsule of right eyes was taken in all the rabbits. Western blotting and reverse transcription polymerase (RT-PCR) were performed to detect survivin expression in PCO at different time points postoperatively. Results Both Western blotting and RT-PCR method indicated that different level of survivin expression was detected in the tissue of PCO 3, 7, 14 and 28 days after surgery. It reached the peak at the 7th day, and began to decrease at the 14th day after surgery. Conclusion The survivin expression can be detected in the tissue of PCO in certain time, which indicated that survivin is correlated with the pathogenesis of PCO.

Key words: Survivin regulatory gene, New Zealand white rabbits, Posterior capsule opacification

CLC Number: 

  • R776.1
[1] Wilhelnus K R, Emery J M. Posterior capsule opacification following phacoemulsification[J]. Ophthalmic Surg, 1980, 11(4):264-267.
[2] Ambrosini G, Adida C, Alteri D C. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphpma[J]. Nat Med, 1997, 3(8):917-921.
[3] Nikolovska-Coleska Z, Meagher J L, Jiang S, et al. Interaction of a cyclic, bivalent smac mimetic with the x-linked inhibitor of apoptosis protein[J]. Biochemistry, 2008, 47(37):9811-9824.
[4] Jiang L B, Liu X Q, Li B, et al. Heat shock proteins and survivin: relationship and effects on proliferation index of retinoblastoma cells[J]. Histol Histopathol, 2008, 23(7):827-831.
[5] Abu-El-Asrar A M, Dralands L, Missotten L, et al. Expression of apoptosis markers in the retinas of human subjects with diabetes[J]. Invest Ophthalmol Vis Sci, 2004, 45(8):2760-2766.
[6] Eldred J A, Hodgkinson L M, Rawes I J, et al. MMP2 activity is critical for TGFβ2-induced matrix contraction-implications for fibrosis[J]. Invest Ophthalmol Vis Sci, 2012, 53(7):4085-4098.
[7] Yeger H, Perbal B. The CCN family of genes: a perspective on CCN biology and therapeutic potential[J]. J Cell Commun Signal, 2007, 1(3-4):159-164.
[8] Soundararajan P, Fawcett J P, Rafuse V F. Guidance of postural motoneurons requires MAPK/ERK signaling downstream of fibroblast growth factor receptor1[J]. J Neurosci, 2010, 30(19):6595-6606.
[9] Iyengar L, Patkunanathan B, McAvoy J W, et al. Growth factors involved in aqueous humour-induced lens cell proliferation[J]. Growth Factors, 2009, 27(1):50-62.
[10] 杨晋, 卢奕.白内障术后后囊膜混浊的基因治疗进展[J].眼科新进展, 2007, 27(5):393-395.
[11] Takehiko D, Elena B, Nathan R W, et al. Mitochondral suivivin inhibits apoptosis and promotes tumorigenesis[J]. J Clin Invest, 2004, 114(8):1117-1127.
[12] Lovicu F J, Schulz M W, Hales A M. TGFbeta induces morphological and molecular change similar to human anterior subcapsular cataract[J]. Br Ophthalmol, 2002, 86(2):220-226.
[13] Yadav U C, Lghani-Hosseinabad F, Van Kuijk F J, et al. Prevention of posterior capsular opacification through aldose reductase inhibition[J]. Invest Ophthalmol Vis Sci, 2009, 50(2):752-759.
[14] 朱洪燕, 蔡小军, 李青春.老年核性及皮质性白内障晶状体上皮细胞中bFGF及α-SMA的表达[J].眼科新进展, 2008, 28(1):33-36.
[1] WANG Jian-min1, LI Jian-ping2, LI Lin-lin3, LIU Jie-hui2, ZHENG Min2. Effect of YAG laser on corneal endothelial cellsin posterior capsule opacification [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(2): 87-89.
[2] WANG Jing,LIU Jing,XIAO Ying,LI Jing-hai,WANG Lei,SUN Hong-yi . Risk factors for the posterior capsule opacification after cataract surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(3): 263-266 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd