JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2014, Vol. 28 ›› Issue (4): 81-84.doi: 10.6040/j.issn.1673-3770.0.2013.172

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Expression of survivin in animal model of posterior capsule opacification

LIU Shu-jun1, DAI Xiu-yu1, LIU Shao-yi1, WANG Wen-ting2, LI Yuan-bin1   

  1. 1. Department of Ophthalmology, Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264000, Shandong, China;
    2. Central Laboratory, Yantai Yuhuangding Hospital, Medical College of Qingdao University, Yantai 264000, Shandong, China
  • Received:2013-06-13 Online:2014-08-16 Published:2014-08-16

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Abstract: Objective To establish animal model of posterior capsule opacification (PCO) in New Zealand white rabbits and detect survivin expression in PCO tissue. Methods Thirty adult New Zealand white rabbits were randomly divided into control group and experimental group. The 5 rabbits in control group were executed. Ultrasonic phacoemulsification was performed in the 25 experimental rabbits' eyes. Their eyes were examined by slit lamp microscope to observe the development of PCO immediately and 3, 7, 14 and 28 days after surgery, and then they were executed. The posterior capsule of right eyes was taken in all the rabbits. Western blotting and reverse transcription polymerase (RT-PCR) were performed to detect survivin expression in PCO at different time points postoperatively. Results Both Western blotting and RT-PCR method indicated that different level of survivin expression was detected in the tissue of PCO 3, 7, 14 and 28 days after surgery. It reached the peak at the 7th day, and began to decrease at the 14th day after surgery. Conclusion The survivin expression can be detected in the tissue of PCO in certain time, which indicated that survivin is correlated with the pathogenesis of PCO.

Key words: Survivin regulatory gene, New Zealand white rabbits, Posterior capsule opacification

CLC Number: 

  • R776.1
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