JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (3): 16-19.doi: 10.6040/j.issn.1673-3770.0.2015.087

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Cytocompatibility research of schwann cells and neural stem cells co-seeded on electron spinning chitosan-PLGA

MEN Yongzhi, CHENG Yusheng, LI Yu, DONG Pin, YU Ziwei   

  1. Department of Otolaryngology & Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai 200080, China
  • Received:2015-02-10 Revised:2015-04-10 Published:2015-06-16

Abstract: Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells (SCs)and neural stem cells(NSCs) as well as cells adhesion and migration on the arranged nanofibers inside the lumen. Methods Experiment was divided into three groups: the SCs group, the NSCs group and the SCs-NSCs co-culture group. Cells in these groups were co-seeded with Chitosan-PLGA. The biocompatibility and absorbability of SCs and NSCs were detected by MTT assays. Scanning electron microscopy(SEM) was used to detect cells adhesion and migration on the axially arranged nanofibers inside the lumen. Results Cell survival rate of the co-culture group was the highest, the higher in the SCs group, and the lowest in the NSCs group. SCs and NSCs had good compatibility and adhesion with Chitosan-PLGA, and the axial nanofibers in the lumen guided the growth of neurites in a particular direction. Conclusion Co-culture of SCs and NSCs can facilitate cells compatibility and adhesion with 3D Chitosan-PLGA, and the axial arranged nanofibers within the lumen has the effect of directional guide cell growth and migration.

Key words: Electron spinning, Chitosan-PLGA, Schwann cells, Neural stem cells, Directional growth

CLC Number: 

  • R745.1
[1] Rezwan K, Chen Q Z, Blaker J J, et al. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering[J]. Biomaterials, 2006, 27(18):3413-3431.
[2] Yang J, Shi G, Bei J, et al. Fabrication and surface modification of macroporous poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture[J]. J Biomed Mater Res, 2002, 62(3):438-446.
[3] Chen Y S, Hsieh C L, Tsai C C, et al. Peripheral nerve regeneration using silicone rubber chambers filled with collagen, laminin and fibronectin[J]. Biomaterials, 2000, 21(15):1541-1547.
[4] Sorenson E J, Windebank A J. Relative importance of basement membrane and soluble growth factors in delayed and immediate regeneration of rat sciatic nerve[J]. J Neuropathol Exp Neurol, 1993, 52(3):216-222.
[5] Schmalenberg K E, Uhrich K E. Micropatterned polymer substrates control alignment of proliferating Schwann cells to direct neuronal regeneration[J]. Biomaterials, 2005, 26(12):1423-1430.
[6] Rodriguez A M, Pisani D, Dechesne C A, et al. Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse[J]. J Exp Med, 2005, 201(9):1397-1405.
[7] Gardner R L. Stem cells and regenerative medicine: principles, prospects and problems[J]. C R Biol, 2007, 330(6-7):465-473.
[8] 沈华, 沈尊理, 陈南樑, 等.不同浓度壳聚糖涂层PLGA材料与许旺细胞相容性研究[J].中国美容医学, 2009, 18(11):1627- 1631. SHEN Hua, SHEN Zunli, CHEN Nanliang, et al. The cytocompatibility of PLGA scaffolds coated with different concentration of chitosan with schwann cells in vitro[J]. Chin J Aesth Med, 2009, 18(11):1627- 1631.
[9] Alessandri G, Emanueli C, Madeddu P. Genetically engineered stem cell therapy for tissue regeneration[J]. Ann N Y Acad Sci, 2004, 1015:271-284.
[10] Goldman S A, Sim F. Neural progenitor cells of the adult brain[J]. Novartis Found Symp, 2005, 265:p. 66-80; discussion 82-97.
[11] Xia, L, Wan H, Hao S Y , et al. Co-transplantation of neural stem cells and Schwann cells within poly (L-lactic-co-glycolic acid) scaffolds facilitates axonal regeneration in hemisected rat spinal cord[J]. Chin Med J, 2013, 126(5):909-917.
[12] Zhang X, Zeng Y, Zhang W, et al. Co-transplantation of neural stem cells and NT-3-overexpressing Schwann cells in transected spinal cord[J]. J Neurotrauma, 2007, 24(12):1863-1877.
[13] Zeng Y S, Ding Y, Wu L Z, et al. Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord[J]. Dev Neurosci, 2005, 27(1):20-26.
[14] Xu L, Zhou S, Feng G Y, et al. Neural stem cells enhance nerve regeneration after sciatic nerve injury in rats[J]. Mol Neurobiol, 2012, 46(2):265-274.
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