JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (6): 56-59.doi: 10.6040/j.issn.1673-3770.0.2015.215

Previous Articles     Next Articles

The experiment of SIRT1 on against oxidative stress to retinal pigmented epithelium cells

LI Langen1, WEI Wei1, ZHANG Yufeng1, Geriletu1, YANG Jia2, ZHANG Yanmei3   

  1. 1. Departments of Ophthalmology, Inner Mongolia People's Hospital, Hohhot 010017, Inner Mongolia, China;
    2. Departments of Neurology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia, China;
    3. Departments of Neurology, Inner Mongolia People's Hospital, Hohhot 010017, Inner Mongolia, China
  • Received:2015-06-02 Online:2015-12-16 Published:2015-12-16

PDF (PC)

349

Abstract: Objective To observe the protection role of the sirtuin 1 (SIRT1) towards the retinal pigmented epithelium(RPE) cells following exposure to oxidative stress. Methods The rates of proliferation and apoptosis, levels of intracellular reactive oxygen species(ROS) and cell senescence of RPEs, induced by oxidants (H2O2), were evaluated. Results The results revealed a down-regulation of SIRT1 expression during oxidative stress. Furthermore, SIRT1 activator resveratrol (RSV) and inhibitors nicotinamide (NA) were respectively treated on RPE cells following exposure by H2O2 intervention; the expression of SIRT1 gene were detected by RT-PCR technology after RNA interfering on SIRT1. The experiments indicated that cell proliferation, apoptosis and ROS levels were significantly different compared with the control group after intervention by H2O2, and cell senescence were significantly increased; NA was significantly increased the toxicity of H2O2, however RSV had the opposite anti-cytotoxicity of H2O2; RT-PCR analysis showed that SIRT1 levels were significantly reduced. Conclusion These results therefore suggested that down-regulation of SirT1 expression during oxidative stress, further investigation indicated that SIRT1 protected RPEs from oxidative stressinduced damage. Appropriate increase of the activity of SIRT1 may be a therapeutic strategy to delay AMD.

Key words: H2O2, Cell growth, Silent information regulator of transcription 1, Retinal pigmented epithelium cells, Reactive oxygen species, siRNA, Oxidative stress

CLC Number: 

  • R774
[1] Beatty S, Koh H, Phil M, et al. The role of oxidative stress in the pathogenesis of agerelated macular degeneration[J]. Surv Ophthalmol, 2000, 45(2):115-134.
[2] Congdon N G, Friedman D S, Lietman T. Important causes of visual impairment in the world today[J]. JAMA, 2003, 290(15):2057-2060.
[3] Kook D, Wolf A H, Yu A L, et al. The protective effect of quercetin against oxidative stress in the human RPE in vitro[J]. Invest Ophthalmol Vis Sci, 2008, 49(4):1712-1720.
[4] Horio Y, Hayashi T, Kuno A, et al. Cellular and molecular effects of sirtuins in health and disease[J]. Clin Sci (Lond), 2011, 121(5):191-203.
[5] Raynes R, Brunquell J, Westerheide S D. Stress inducibility of SIRT1 and its role in cytoprotection and cancer[J]. Genes Cancer, 2013, 4(3-4):172-182.
[6] Alcendor R R, Gao S, Zhai P, et al. Sirt1 regulates aging and resistance to oxidative stress in the heart[J]. Circ Res, 2007, 100(10):1512-1521.
[7] Kawai Y, Garduno L, Theodore M, et al. Acetylation-deacetylation of the transcripttion factor Nrf2 (nuclear factor erythroid 2-related factor 2) regulates its transcriptional activity and nucleocytoplasmic localization[J]. J Biol Chem, 2011, 286(9):7629-7640.
[8] Khan J C, Thurlby D A, Shahid H, et al. Smoking and age related macular degeneration: the number of pack years of cigarette smoking is a major determinant of risk for both geographic atrophy and choroidal neovascularisation[J]. Br J Ophthalmol, 2006, 90(1): 75-80.
[9] AgeRelated Eye Disease Study Research Group. A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E and beta carotene for agerelated cataract and vision loss: AREDS report no.9[J]. Arch Ophthalmol, 2001, 119(10):1439-1452.
[10] Cai J, Nelson K C, Wu M, et al. Oxidative damage and protection of the RPE[J]. Prog Retin Eye Res, 2000, 19(2):205-221.
[11] Li Z, Dong X, Liu H, et al. Astaxanthin protects ARPE-19 cells from oxidative stress via upregulation of Nrf2-regulated phase II enzymes through activation of PI3K/Akt[J]. Mol Vis, 2013, 19:1656-1666.
[12] Guarani V, Deflorian G, Franco C A, et al. Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase[J]. Nature, 2011, 473(7346):234-238.
[1] ZHANG Zhuan, LIU Tao, BAI Zhili, ZHOU Changming. Evolution of oxidative stress in the pathogenesis and treatment of noise-induced hearing loss. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 101-103.
[2] . Effects of rosiglitazone on oxidative stress and cognitive function in mice exposed to intermittent hypoxia. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 45-49.
[3] LI Yanzhong. Obesity and obstructive sleep apnea. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 1-4.
[4] DU Xiangge, ZHANG Yingchun, YAN Xin, WANG Cui, ZHAO Bojun. In vitro study of the effect of Casein Kinase2 siRNA on anginogesis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(3): 76-80.
[5] LIU Hong, WANG Shuai, WANG Hai-bo. Review on mtDNACD4977 and presbyacusis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(4): 95-99.
[6] TANG Zhi, CHEN Yong-Ming, WANG Ji-Qun. Effect of siRNA by targeting the human hTERT gene on morphology in Hep-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(5): 4-7.
[7] BI Hong-sheng,LI Shu-jie,CUI Yan,WANG Hui . Effect of tea polyphenols in preventing diabetic cataracts induced by streptozotocin in rats and its mechanism [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(1): 1-05 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd