miR-30-5p通过下调FOXG1表达抑制视网膜母细胞瘤细胞增殖

doi:10.6040/j.issn.1673-3770.0.2021.466

Abstract

Abstract: Objective To investigate the effect of microRNA-30-5p(miR-30-5p)on the proliferation of retinoblastoma cells. Methods The expression of miR-30-5p in retinoblastoma cell lines and normal human retinal epithelial cells(HRECs)was determined using quantitative Real-time polymerase chain reaction(qRT-PCR). Bioinformatic analysis and prediction of miR-30-5p target genes were performed using the TargetScan database. Dual-luciferase reporter experiments verified the 3'-UTR binding ability and targeting relationship of miR-30-5p and FOXG1. The effect of miR-30-5p on the mRNA and protein expression of FOXG1 was determined using qRT-PCR and Western blotting, respectively. The effects of the miR-30-5p-FOXG1 pathway on cell proliferation were examined in Y79 cells by transient transfection. Results miR-30-5p expression was decreased and FOXG1 expression was increased in retinoblastoma cells compared with that in HRECs. Bioinformatics prediction results revealed the binding sites of miR-30-5p and FOXG1. qRT-PCR and western blot results showed that miR-30-5p negatively regulated FOXG1 mRNA and protein expression. Dual-luciferase assay confirmed a targeting relationship between miR-30-5p and FOXG1 3'-UTR. Transient transfection of miR-30-5p increased the expression level of miR-30-5p in Y79 cells. Overexpression of FOXG1 reversed the inhibition of Y79 cell proliferation. Conclusion miR-30-5p inhibits the proliferation of Y79 retinoblastoma cells by downregulating FOXG1 expression.

Key words: Retinoblastoma, Y79, miR-30-5p, FOXG1, Cell proliferation, Human retinal epithelial cells

CLC Number:

R774.1

YAN Fancheng, JIANG Xian, CHAI Yijie, WANG Haosen, MENG Zhaoyang, WANG Xiaolei, WANG Yanling,. miR-30-5p inhibits retinoblastoma cell proliferation by downregulating FOXG1 expression[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 63-69.

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miR-30-5p inhibits retinoblastoma cell proliferation by downregulating FOXG1 expression

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