Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (4): 105-108.doi: 10.6040/j.issn.1673-3770.0.2018.132

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Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress

SU Jie, MA Chunmei, ZHAO Lili, LIU Yan, SHAO Hongchao   

  1. Department of Ophthalmology, Affiliated Hospital of North China University of Technology, Tangshan 063000, Hebei, China
  • Online:2019-07-20 Published:2019-07-22

Abstract: Objective To evaluate miR-204 expression in human retinoblastoma and to investigate the effect of latter's growth and molecular mechanism. Methods RT-PCR was performed to detect the expression of miR-204 in retinoblastoma(Y79)and the normal retinal(ARPE-19)cell lines. The mimic(s)and inhibitor(s)of miR-204 were transiently transfected into Y79 cells. CCK-8 and flow cytometry aided the detection of the survival and apoptosis of Y79 cells. The expression level of GRP78 protein was determined by Western blot. Results The expression of miR-204 in Y79 cells was significantly lower than that in ARPE-19. It was observed that the over-expressed miR-204 in Y79 cells could significantly inhibit the proliferation of retinoblastoma. Moreover, it promoted apoptosis, increased GRP78 protein's expression, and could activate the endoplasmic reticulum stress pathway. Conclusion miR-204 expression in retinoblastoma cells was significantly lower than that in the normal retinal cells. In addition, miR-204 might induce retinoblastoma cell(s)apoptosis by activating the endoplasmic reticulum stress pathway.

Key words: miR-204, Retinoblastoma, Endoplasmic reticulum stress

CLC Number: 

  • R774.1
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