miR-204通过内质网应激抑制视网膜母细胞瘤生长作用及机制的研究

doi:10.6040/j.issn.1673-3770.0.2018.132

Abstract

Abstract: Objective To evaluate miR-204 expression in human retinoblastoma and to investigate the effect of latter's growth and molecular mechanism. Methods RT-PCR was performed to detect the expression of miR-204 in retinoblastoma(Y79)and the normal retinal(ARPE-19)cell lines. The mimic(s)and inhibitor(s)of miR-204 were transiently transfected into Y79 cells. CCK-8 and flow cytometry aided the detection of the survival and apoptosis of Y79 cells. The expression level of GRP78 protein was determined by Western blot. Results The expression of miR-204 in Y79 cells was significantly lower than that in ARPE-19. It was observed that the over-expressed miR-204 in Y79 cells could significantly inhibit the proliferation of retinoblastoma. Moreover, it promoted apoptosis, increased GRP78 protein's expression, and could activate the endoplasmic reticulum stress pathway. Conclusion miR-204 expression in retinoblastoma cells was significantly lower than that in the normal retinal cells. In addition, miR-204 might induce retinoblastoma cell(s)apoptosis by activating the endoplasmic reticulum stress pathway.

Key words: miR-204, Retinoblastoma, Endoplasmic reticulum stress

CLC Number:

R774.1

SU Jie, MA Chunmei, ZHAO Lili, LIU Yan, SHAO Hongchao. Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 105-108.

References 13

[1]	赵堪兴, 杨培增. 眼科学[M]. 8版. 北京: 人民卫生出版社, 2013.
[2]	Ma L, Deng XB, Wu MH, et al. Down-regulation of miRNA-204 by LMP-1 enhances CDC42 activity and facilitates invasion of EBV-associated nasopharyngeal carcinoma cells[J]. FEBS Lett, 2014, 588(9): 1562-1570. doi:10.1016/j.febslet.2014.02.039.
[3]	Chintagumpala M, Chevez-Barrios P, Paysse EA, et al. Retinoblastoma: review of current management[J]. Oncologist, 2007, 12(10): 1237-1246. doi:10.1634/theoncologist.12-10-1237.
[4]	Han JJ, Lee Y, Yeom KH, et al. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex[J]. Cell, 2006, 125(5): 887-901. doi:10.1016/j.cell.2006.03.043.
[5]	蒋成义, 汪洪涛, 周蕾, 等. microRNA-204在鼻咽癌中的表达及生物学意义研究[J]. 中国免疫学杂志, 2015, 31(6): 822-826. doi:10.3969/j.issn.1000-484X.2015.06.023. JIANG Chengyi, WANG Hongtao, ZHOU Lei, et al. Expression and biological significance of microRNA-204 in nasopharyngeal carci-noma[J]. Chinese Journal of Immunology, 2015, 31(6): 822-826. doi:10.3969/j.issn.1000-484X.2015.06.023.
[6]	Klase Z, Houzet L, Jeang KT. Replication competent HIV-1 viruses that express intragenomic microRNA reveal discrete RNA-interference mechanisms that affect viral replication[J]. Cell Biosci, 2011, 1(1): 38. doi:10.1186/2045-3701-1-38.
[7]	Li WD, Jin XJ, Zhang QB, et al. Decreased expression of miR-204 is associated with poor prognosis in patients with breast cancer[J]. Int J Clin Exp Pathol, 2014, 7(6): 3287-3292.
[8]	杨林声, 王理论, 杜青卫. miR-204通过下调Bcl-2表达抑制人视网膜母细胞瘤细胞生长[J]. 现代肿瘤医学, 2016, 24(2): 208-212. YANG Linsheng, WANG Lilun, DU Qingwei. Growth inhibition of human retinoblastoma by miR-204 via down- regulation of Bcl-2 expression[J]. Journal of Modern Oncology, 2016, 24(2): 208-212.
[9]	Zhang LH, Zhang X. Roles of GRP78 in physiology and cancer[J]. J Cell Biochem, 2010, 110(6): 1299-1305. doi:10.1002/jcb.22679.
[10]	Mikoshiba K. IP3 receptor/Ca2+ channel: from discovery to new signaling concepts[J]. J Neurochem, 2007, 102(5): 1426-1446. doi:10.1111/j.1471-4159.2007.04825.x.
[11]	王新, 范立君, 李壮, 等. GRP78、GRP94在乳腺癌中的表达及其意义[J]. 黑龙江医药科学, 2014, 37(6): 89-91. doi:10.3969/j.issn.1008-0104.2014.06.049.
[12]	曹雪霞. GRP78在人肝细胞癌组织中的表达及临床意义[D]. 郑州: 郑州大学, 2013.
[13]	Guo ZY, Maki M, Ding RF, et al. Genome-wide survey of tissue-specific microRNA and transcription factor regulatory networks in 12 tissues[J]. Sci Rep, 2014, 4: 5150. doi:10.1038/srep05150.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	YANG Changliang,HUANG Zhiwu,YAO Hangqi,ZHU Yong,SNU Yi . Study on auditory brainstem response[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 9 -13 .
[2]	. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 89 -89 .
[3]	LIU Da-yu,PAN Xin-liang,LEI Da-peng,ZHANG Li-qiang,LUAN Xin-yong . Surgical treatment for medial wall pyriform sinus cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2007, 21(1): 8 -11 .
[4]	. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(2): 188 -188 .
[5]	LIU Yan,LIU Xin-yi,WANG Jin-ping,LI Da-jian . Measurement of the posterior tympanum and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 218 -221 .
[6]	ZHAO Min,WANG Shou-sen,ZHEN Ze-nian,CHEN Xian-ming,WANG Mao-xin . Sphenoid sinus and trans-sphenoid surgery under nasal endoscopy and microscopy [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 244 -245 .
[7]	. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 252 -252 .
[8]	HUANG Fang,HUANG Hai-qiong,HUANG Jian-qiang,HE He-fan . Bronchoscopic video supervision system in infant bronchial foreign bodies[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 276 -277 .
[9]	YU Zhi-liang,WANG Wei-wei,WANG Ming-hua . Excision of huge epiglottis cysts under MedtrnicXPS3000 in 23 cases[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 278 -279 .
[10]	ZHANG Jian-li,WANG Yue-jian,CHEN Wei-xiong,HU Wei-wei,LI Guang-min . Immunohistochemistry and serial section in detection of lymph node micrometastasis of head and neck squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(4): 299 -303 .

Inhibitory effect of miR-204 and the molecular mechanism of RB via endoplasmic reticulum stress

PDF (PC)

Abstract

Cite this article

share this article

References 13

Related Articles 2

Metrics

Comments

Recommended 10

[1]	TENG Xingbo, CAO Zhi, SUN Haixia, LIU Xianjin, YANGa Weizhou, ZHU Yan, ZHU Yuguang. Role of GRP94 and EIF2α in trabecular bone of primary angle-closure glaucoma patients [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 115-118.
[2]	WANG Hong-xia,WANG Peng-cheng . Expression of NSE,S100 and GFAP in retinoblastoma and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 263-264 .