Abstract:

To study the effect of pSilencer2.0cMetsiRNA on the growth of tumors after transplantation from human cancer of larynx Hep2 into nude mice. MethodsHep2 cells were transplanted into nude mice and the time of tumor formation and growth were observed. After tumor formation, cMetsiRNA was given as the antitumor therapy. The tumor volume was calculated and the tumor growth curve was plotted. Representative histological sections were taken from mice bearing transplantation tumors in either experimental or control groups; Expression of cMet, MMP2, MMP9 and VEGF were detected by Western blot method. ResultsOn the 35th day after tumor vaccination, the tumor volume was 13?827?mm3 in the cMetsiRNA transfection group, which was diminished significantly in contrast with the control group（P＜0.01）. Expression of cMet, MMP2, MMP9 and VEGF in the tumors of the experimental group was decreased significantly(P＜0.05). ConclusionspSilencer2.0cMetsiRNA can significantly inhibit the growth of transplantation tumors of nude mice from human cancer of the larynx, and siRNA expressing plasmid mediated gene therapy may be a new strategy in targeting molecular therapy of cancer of the larynx.

Key words: Short hairpin RNA; cMet gene; Larynx neoplasms