Abstract:

Objective     To explore the effects of macrophage inflammatory protein-3α/CCL20 production by interleukin-17 in nasal polyp epithelial cells. Methods        Epithelial cell lines,which were established from nasal polyps, were stimulated by culture medium, IL-17.The amount of MIP-3α/CCL20 in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA). MIP-3α/CCL20 mRNA expression was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Results    Expression levels of MIP-3α/CCL20 in nasal polyps were significantly increased compared with those only in the culture medium. IL-17 induced MIP-3α/CCL20 production by nasal polyp epithelial cells in a dose- and time-dependent manner. Conclusions    Nasal polyp epithelial cells, by producing MIP-3α/CCL20, which can be induced by Interleukin-17, may play an important role in the recruitment of T cells and DCs in nasal polyps.

Key words: Nasal polyps; IL-17; MIP-3α/CCL20