Table of Content

25 April 2006 Volume 20 Issue 2

  

Articles

Cloning and identification of human granulocytemacrophage

LI Minxiong,CHEN Shengqiang,LIU Qicai,ZHANG Jianguo

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  105-107 . 

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［ABSTRACT］Objective: To construct recombinant granulocytemacrophage colonystimulating factor （GMCSF） retrovirus expressing vector by cloning of GMCSF genes from human blood and construct the recombinant plasmids encoding for GMCSF. Methods: Primers for GMCSF were designed and synthesized according to the sequences of human GMCSF genes derived from GenBank. The full length cDNA of GMCSF was cloned by RTPCR techniques. The recombinant plasmids pGEMTGMCSF were constructed by recombinant gene techniques. Results: The length of RTPCR product coincided with that of authors′ anticipation(456bp)，and the recombinant plasmid was confirmed by XbaⅠ /NotⅠ restriction enzyme digesting. The sequencing result of the cDNA was identical to the sequence of GMCSF cDNA in GenBank, and the full length cDNA of human GMCSF was successfully inserted into the vector of pGEMT. Conclusion: The successful cloning of human GMCSF cDNA, as well as construction of its retrovirus expressing vector enables us to further investigate the role of GMCSF in tumor immunogene therapy.

Clinical and experimental study on the overexcitatory of facial nerve in intratemporal facial paralysis

SHI Lei, LI Xiaotian, REN Zhong

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  108-110 . 

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Objective: To study the manifestation, significance and mechanisms of facial nerve overexcitatory in facial paralysis. Methods： The normal threshold of the excitatory of facial nerve was tested in 100 normal persons by nerveexcitatory testing machine. The excitatory of 190 patients suffering from facial paralysis was also tested. The experimental model of facial paralysis was made in guinea pigs. Electromyograph was tested and the changes of myelin sheath of nerve fibers were observed by transmission electron microscope. The cure duration of the over and none overexcitatory group was compared by ttest. Results: The normal threshold of excitatory of facial nerve was 1.72～-1.57 mA in normal person. Of the 190 patients suffering from facial paralysis, 29 showed overexcitatory. It took (23.4±6.0)days for the overexcitatory group to be clinically cured, while it took (42.6±11.2)days for the none overexcitatory group(P＜0.01). The threshold of 8 guinea pigs decreased after compression in 23 guinea pigs showing overexcitatory. Slight separation of myelin sheath layer of nerve fibers was observed by electron microscopy. Conclusion: The prognosis of facial paralysis with overexcitatory is good. Overexcitatory and separation of myelin sheath make the amount of ions that increase the excitatory of nerve.

Correlation between prognosis and increase of leucocyte count for the sudden hearing loss

ZHANG Ming,ZHONG Gang-yi

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  111-113 . 

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Objective: To study the correlation between the prognosis and leucocyte count in sudden hearing loss. Method： A total of 135 cases of sudden hearing loss were retrospectively analyzed. Results： The increase of leucocytes had no correlation with the auditory threshold volume（P＞0.05）. The curative rate in sudden hearing loss patients with normal leucocyte number was higher than that in patients with increased leucocyte and neutrophil peripheral leucocyte levels. Conclusion: The treatment efficiency for patients with sudden hearing loss with increased leucocyte level is higher than that for patients with normal leucocyte level.

Ixeris sonchifolia Hance injection for sudden deafness

LI Yong-xiang,ZHANG Wu-ning,HUANG Jian-cheng,WU Cheng-lin

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  114-115 . 

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Objective: To study the clinical value of ixeris sonchifolia Hance injection in the treatment of sudden deafness. Methods: One hundred and twenty patients were averagely divided into two groups randomly. The treatment group was given ixeris sonchifolia Hance injection, and control group both low molecular dextran and compound Danshen injection. Results: The total response rates for deafness and tinnitus were 88.33% and 82.86% in the treatment group, and 68.33% and 62.50% in the control group(bothP＜0.05). Conclusion: Ixeris sonchifolia Hance injection is effective and safe in the treatment of sudden deafness.

Revision endoscopic sinus surgery on chronic sinusitis and polyps

ZHENG Guojun,TONG Shuhong,WANG Qiaofang,YANG Dun

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  124-125 . 

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［ABSTRACT］Objective: To explore the effect of reoperation for recurrent sinusitis and polyps. Methods: Reoperation by endoscope was performed in 46 patients, of which Messerklinger′s technique was used in 32, and Wigand′s technique was used in 14. Results: Reoperation was successful in 39 patients (78.3%), but not in 10 (21.7%). Complication was found in 1 patient. Conclusion: Revision endoscopic sinus surgery is effective for recurrent sinusitis and polyps. CT scanning has salutary effect for reducing complications.

Radio frequency for refractory epistaxis of small vessel expansion on nasal septum

WANG Yansheng,GONG Zhenyang,ZHU Chunsheng,ZHOU Suping

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  130-131 . 

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ABSTRACT］Objective： To study the diagnosis of obstinate epistaxis of small vessel expansion of nasal septum and the management by radio frequency. Method： The data of 38 patients with obstinate epistaxis of small vessel expansion of nasal septum were reviewed. Results： All patients had been treated successfully by radio frequency and followed up over 6 months. No complication due to treatment, such as perforation of nasal septum and so on occurred, and no nasal bleeding recurred during the followup. Conclusion： Radio frequency, performed under local surface anaesthesia, is effective in treating intractable epistaxis of small vessel expansion of nasal septum.

YUE Lihua， CHENG Jinmei， LIN Gongbiao， CHEN Guohao, ZHANG Rong, YI Zixiang

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2):  135-138 . 

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［ABSTRACT］Objective： To discuss the diagnosis, treatment and prognosis of the primary nonHodgkin lymphoma in nose, pharynx and larynx. Methods： The clinical data of 64 primary nasal, pharyngeal and laryngeal nonHodgkin lymphomas were retrospectively reviewed. The prognostic factors of the lymphoma were evaluated. Results： Of the 64 patients, 29 had T cell lymphomas, 16 NK/T cell lymphomas, 16 B cell lymphomas and 3 unclassified lymphomas; of the 31 patients of Ann Arbor stage, 21 were stageⅡ, 11 stage Ⅲand 1 stage Ⅳ; 25 patients received chemotherapy alone, 27 chemotherapy combined with radiotherapy, and 5 radiotherapy alone. The 3year overall survival rate was 67.24%. There was no significant difference of the 3year survival rate between the chemotherapy and the chemotherapy combined with radiotherapy (P=0.0704). Univariate analysis showed that Ann Arbor classification, international prognostic index (IPI), performance state and LDH were prognostic factors. Multivariate analysis by Cox regression demonstrated that only IPI was an independent prognostic factor. Conclusion： The T cell lymphoma is the predominant type of NHL in nose, pharynx and larynx. The therapy of chemotherapy combined with radiotherapy can not improve the cure rate. IPI is an independent prognostic factor.