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25 April 2006 Volume 20 Issue 2
Articles
Cloning and identification of human granulocytemacrophage
LI Minxiong,CHEN Shengqiang,LIU Qicai,ZHANG Jianguo
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 105-107 .
Abstract
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[ABSTRACT]Objective: To construct recombinant granulocytemacrophage colonystimulating factor (GMCSF) retrovirus expressing vector by cloning of GMCSF genes from human blood and construct the recombinant plasmids encoding for GMCSF. Methods: Primers for GMCSF were designed and synthesized according to the sequences of human GMCSF genes derived from GenBank. The full length cDNA of GMCSF was cloned by RTPCR techniques. The recombinant plasmids pGEMTGMCSF were constructed by recombinant gene techniques. Results: The length of RTPCR product coincided with that of authors′ anticipation(456bp),and the recombinant plasmid was confirmed by XbaⅠ /NotⅠ restriction enzyme digesting. The sequencing result of the cDNA was identical to the sequence of GMCSF cDNA in GenBank, and the full length cDNA of human GMCSF was successfully inserted into the vector of pGEMT. Conclusion: The successful cloning of human GMCSF cDNA, as well as construction of its retrovirus expressing vector enables us to further investigate the role of GMCSF in tumor immunogene therapy.
Clinical and experimental study on the overexcitatory of facial nerve in intratemporal facial paralysis
SHI Lei, LI Xiaotian, REN Zhong
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 108-110 .
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Objective: To study the manifestation, significance and mechanisms of facial nerve overexcitatory in facial paralysis. Methods: The normal threshold of the excitatory of facial nerve was tested in 100 normal persons by nerveexcitatory testing machine. The excitatory of 190 patients suffering from facial paralysis was also tested. The experimental model of facial paralysis was made in guinea pigs. Electromyograph was tested and the changes of myelin sheath of nerve fibers were observed by transmission electron microscope. The cure duration of the over and none overexcitatory group was compared by ttest. Results: The normal threshold of excitatory of facial nerve was 1.72~-1.57 mA in normal person. Of the 190 patients suffering from facial paralysis, 29 showed overexcitatory. It took (23.4±6.0)days for the overexcitatory group to be clinically cured, while it took (42.6±11.2)days for the none overexcitatory group(P<0.01). The threshold of 8 guinea pigs decreased after compression in 23 guinea pigs showing overexcitatory. Slight separation of myelin sheath layer of nerve fibers was observed by electron microscopy. Conclusion: The prognosis of facial paralysis with overexcitatory is good. Overexcitatory and separation of myelin sheath make the amount of ions that increase the excitatory of nerve.
Correlation between prognosis and increase of leucocyte count for the sudden hearing loss
ZHANG Ming,ZHONG Gang-yi
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 111-113 .
Abstract
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2671
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Objective: To study the correlation between the prognosis and leucocyte count in sudden hearing loss. Method: A total of 135 cases of sudden hearing loss were retrospectively analyzed. Results: The increase of leucocytes had no correlation with the auditory threshold volume(P>0.05). The curative rate in sudden hearing loss patients with normal leucocyte number was higher than that in patients with increased leucocyte and neutrophil peripheral leucocyte levels. Conclusion: The treatment efficiency for patients with sudden hearing loss with increased leucocyte level is higher than that for patients with normal leucocyte level.
Ixeris sonchifolia Hance injection for sudden deafness
LI Yong-xiang,ZHANG Wu-ning,HUANG Jian-cheng,WU Cheng-lin
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 114-115 .
Abstract
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Objective: To study the clinical value of ixeris sonchifolia Hance injection in the treatment of sudden deafness. Methods: One hundred and twenty patients were averagely divided into two groups randomly. The treatment group was given ixeris sonchifolia Hance injection, and control group both low molecular dextran and compound Danshen injection. Results: The total response rates for deafness and tinnitus were 88.33% and 82.86% in the treatment group, and 68.33% and 62.50% in the control group(bothP<0.05). Conclusion: Ixeris sonchifolia Hance injection is effective and safe in the treatment of sudden deafness.
Revision endoscopic sinus surgery on chronic sinusitis and polyps
ZHENG Guojun,TONG Shuhong,WANG Qiaofang,YANG Dun
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 124-125 .
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[ABSTRACT]Objective: To explore the effect of reoperation for recurrent sinusitis and polyps. Methods: Reoperation by endoscope was performed in 46 patients, of which Messerklinger′s technique was used in 32, and Wigand′s technique was used in 14. Results: Reoperation was successful in 39 patients (78.3%), but not in 10 (21.7%). Complication was found in 1 patient. Conclusion: Revision endoscopic sinus surgery is effective for recurrent sinusitis and polyps. CT scanning has salutary effect for reducing complications.
Radio frequency for refractory epistaxis of small vessel expansion on nasal septum
WANG Yansheng,GONG Zhenyang,ZHU Chunsheng,ZHOU Suping
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 130-131 .
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ABSTRACT]Objective: To study the diagnosis of obstinate epistaxis of small vessel expansion of nasal septum and the management by radio frequency. Method: The data of 38 patients with obstinate epistaxis of small vessel expansion of nasal septum were reviewed. Results: All patients had been treated successfully by radio frequency and followed up over 6 months. No complication due to treatment, such as perforation of nasal septum and so on occurred, and no nasal bleeding recurred during the followup. Conclusion: Radio frequency, performed under local surface anaesthesia, is effective in treating intractable epistaxis of small vessel expansion of nasal septum.
YUE Lihua, CHENG Jinmei, LIN Gongbiao, CHEN Guohao, ZHANG Rong, YI Zixiang
JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2006, 20(2): 135-138 .
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[ABSTRACT]Objective: To discuss the diagnosis, treatment and prognosis of the primary nonHodgkin lymphoma in nose, pharynx and larynx. Methods: The clinical data of 64 primary nasal, pharyngeal and laryngeal nonHodgkin lymphomas were retrospectively reviewed. The prognostic factors of the lymphoma were evaluated. Results: Of the 64 patients, 29 had T cell lymphomas, 16 NK/T cell lymphomas, 16 B cell lymphomas and 3 unclassified lymphomas; of the 31 patients of Ann Arbor stage, 21 were stageⅡ, 11 stage Ⅲand 1 stage Ⅳ; 25 patients received chemotherapy alone, 27 chemotherapy combined with radiotherapy, and 5 radiotherapy alone. The 3year overall survival rate was 67.24%. There was no significant difference of the 3year survival rate between the chemotherapy and the chemotherapy combined with radiotherapy (P=0.0704). Univariate analysis showed that Ann Arbor classification, international prognostic index (IPI), performance state and LDH were prognostic factors. Multivariate analysis by Cox regression demonstrated that only IPI was an independent prognostic factor. Conclusion: The T cell lymphoma is the predominant type of NHL in nose, pharynx and larynx. The therapy of chemotherapy combined with radiotherapy can not improve the cure rate. IPI is an independent prognostic factor.