Objective To analyze the clinical characteristics of the acoustic rhinometry with nasal septum perforation. Methods 18 cases of nasal septal perforation were received acoustic rhinometry before and after surgery and also were evaluated nasal obstruction score by visual analogue scale(VAS) the SPSS16.0 software underwent a t test paired data before and after surgery, also were analyzed the correlation among VAS score, NCV2-7 and NMCA. Results Pre-operative NCV2-7, NMCA, and VAS scores were respectively 8.25±1.12cm3, 0.54±0.08cm2, and 3.46±1.02, post-operative were 6.45±0.98cm3, 0.48±0.07cm2, 1.74±0.64, preoperative, and post-operative NCV2-7 and VAS score showed significantly difference (P<0.01), NMCA showed no significant difference before and after surgery (P>0.05). the correlation coefficients of the pre-operative and post-operative VAS scores and NMCA were 0.762, 0.814, pre-operative and post-operative correlation coefficients of the VAS score and NCV2-7 were 0.384, 0.432, pre-operative and post-operative correlation coefficients of the NCV2-7 and NMCA were 0.234, 0.316, pre-operative and post-operative VAS scores and NMCA's relationship showed statistical significance (P<0.01), pre-operative and post-operative VAS scores and NCV2-7 showed no statistically significant correlation, the correlation between pre-operative and post-operative NMCA and NCV2-7 showed no statistical significance. Conclusion The acoustic rhinometry graph of nasal septum perforation has typical characteristics:Corresponding to the perforated parts of the bilateral nasal acousticrhinometry graph showing significantly higher changes in symmetry, and the symmetry can indirectly reflect some distance from the front to the back perforation, for judging the perforation location, size, forensic, therapeutic decision, finding occult perforation, surgical side of the nasal cavity, surgical approach, operative procedures have certain significance.