JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2014, Vol. 28 ›› Issue (1): 10-13.doi: 10.6040/j.issn.1673-3770.0.2013.286

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Regeneration of motor neurons induced by different concentrations of GDNF in vitro

XIA Si-wen1,2, CHEN Shi-cai1,  HUANG Yi-deng2, LI Meng1, LI Xiao-yu1, ZHENG Hong-liang1   

  1. Department of Otorhinolaryngology & Head and Neck Surgery, 1. Changhai Hospital, The Second Military Medical University, Shanghai 200433, China; 2. The 118th Hospital of PLA， Wenzhou 325000, Zhejiang, China
  • Received:2013-10-10 Online:2014-02-16 Published:2014-02-16

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Abstract:

Objective   To investigate the regeneration of motor neurons induced by different concentrations of glial cell line-derived neurotrophic factor(GDNF) in vitro to determine the optimal GDNF concentration. Methods   With the motor neuron regeneration model, different concentrations of GDNF were added into the chamber and the formation of a stable concentration gradient was achieved. After 72-hour cell culture, the optimal concentration of GDNF to induce the regeneration was determined by measuring the angles and lengths of growth cones on motor neurons under different concentrations. Results   For the regeneration of motor neurons, there was no significant difference between GDNF 200ng/mL group and GDNF 100ng/mL group (P＞0.05), however, both groups showed better effects than the blank control group and GDNF 50ng/mL group (P<0.05).  The difference between the latter two groups was not significant (P>0.05). Conclusion    GDNF could induce the regeneration of motor neurons significantly, and the optimal concentration in vitro is 100ng/mL.

Key words: Glial cell line-derived neurotrophic factor, Induced regeneration, Motor neuron, Concentration gradient

CLC Number: 

  • R764.4
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