Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (2): 41-51.doi: 10.6040/j.issn.1673-3770.0.2023.217

• Original Article • Previous Articles     Next Articles

Elucidation of the Mongolian medicine Huricha-6 mechanism in treating allergic rhinitis via network pharmacology and animal experiments

SU Riguge, LI Hua, WU Richaifu, HAN Eerdemutu, MENG Yongmei   

  1. College of Mongdian Medicine, Inner Mongolia Medical Vniversity, Hohhot 010110, Inner Mongolia, China
  • Online:2024-03-20 Published:2024-03-29

PDF (PC)

199

Abstract: Objective To explore the possible mechanism of Mongolian medicine Huricha-6 in treating allergic rhinitis,based on network pharmacology and animal experiments. Methods Compounds and targets of Huricha-6 were obtained from the TCMSP database and literature. Allergic rhinitis(AR)-related targets were identified via searches of the OMIM, TTD, Disgenet, and GeneCards databases. Then, the Huricha-6 target and AR target intersection was identified by constructing a "compound-intersection target" network and selecting key compounds. The STRING database was used to draw the protein interaction network to screen core targets, and R language was used to conduct gene ontology(GO)and kyto encyclopedia of genes and genomes(KEGG)pathway analysis on intersection targets. AutoDockVina software was used to verify key compounds and targets by molecular docking. An AR guinea pig model was constructed for Huricha-6 testing. Guinea pigs were treated with Huricha-6 for 6 weeks, then their nasal symptoms were evaluated by behavioral assessment. Nasal mucosa tissue was taken for HE staining to observe lesions, and IL-4 and IFN-γ levels were detected by ELISA. Results Network pharmacology analysis identified 107 possible targets for Huricha-6 relevant to AR treatment, and 30 compounds related to Huricha-6 in the treatment of AR. Quercetin, luteolin, kaolin, isorhamnetin and quinine were the key compounds, and the key target include VEGFA, STAT3, IL-1B, IL-6 and ALB. KEGG pathway enrichment revealed involvement of TNF, IL-17, Toll and HIF-1 pathways. Molecular docking supports the premise that key compounds will exhibit strong binding to core targets. Animal experiments demonstrate that Huricha-6 can effectively reduce nasal symptoms, improve pathological changes in the nasal mucosa, significantly reduce serum IL-4 levels, and increase IFN-γ levels. Conclusion Huricha -6 can ameliorate AR symptoms via interactions with multiple targets that act in multiple pathways, may alleviate inflammatory cell infiltration of nasal mucosal tissue, and alleviate AR symptoms by modulating the inflammatory response.

Key words: Mongolian medicine, Huricha-6, Allergic rhinitis, Network pharmacology, Animal experiments

CLC Number: 

  • R765.21
[1] Meng YF, Wang CS, Zhang L. Advances and novel developments in allergic rhinitis[J]. Allergy, 2020, 75(12): 3069-3076. doi:10.1111/all.14586
[2] 倪璟滋, 万文锦. 变应性鼻炎健康相关生活质量研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 110-115. doi:10.6040/j.issn.1673-3770.1.2021.165 NI Jingzi, WAN Wenjin. Research progress on health-related quality of life in allergic rhinitis[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 110-115. doi:10.6040/j.issn.1673-3770.1.2021.165
[3] Naclerio R, Ansotegui IJ, Bousquet J, et al. International expert consensus on the management of allergic rhinitis(AR)aggravated by air pollutants: impact of air pollution on patients with AR: current knowledge and future strategies[J]. World Allergy Organ J, 2020, 13(3): 100106. doi:10.1016/j.waojou.2020.100106
[4] 黄毅芳, 林辰, 邓成海, 等. 民族医药治疗变应性鼻炎的研究概况[J]. 广西中医药大学学报, 2020, 23(3): 49-52. doi:10.3969/j.issn.2095-4441.2020.03.017 HUANG Yifang, LIN Chen, DENG Chenghai, et al. An overview of research on ethnic medicine in treatment of allergic rhinitis[J]. Journal of Guangxi University of Chinese Medicine, 2020, 23(3): 49-52. doi:10.3969/j.issn.2095-4441.2020.03.017
[5] 张燕, 姜昊. 蒙药胡日查-6治疗过敏性鼻炎38例[J]. 中国民族医药杂志, 2006, 12(6): 20. doi:10.16041/j.cnki.cn15-1175.2006.06.014 ZHANG Yan, JIANG Hao. 38 cases of allergic rhinitis treated by Mongolian medicine hu richa-6[J]. Journal of Medicine & Pharmacy of Chinese Minorities, 2006, 12(6): 20. doi:10.16041/j.cnki.cn15-1175.2006.06.014
[6] 高佳丽. 黑云香化学成分的研究[D]. 内蒙古医科大学,2008. doi:10.7666/d.y1292909
[7] 刘淑琴. 黑云香挥发油化学成分GC/MS分析[J]. 内蒙古民族大学学报(自然科学版), 2011, 26(5): 515-517. doi:10.3969/j.issn.1671-0185.2011.05.007 LIU Shuqin. GC-MS analysis of volatile oil components from black rue[J]. Journal of Inner Mongolia Minzu University(Natural Sciences), 2011, 26(5): 515-517. doi:10.3969/j.issn.1671-0185.2011.05.007
[8] 黄庞宁, 杨慧芳, 刘素丽, 等. 基于网络药理学和分子对接方法探讨保元汤治疗扩张型心肌病的作用机制[J]. 中药新药与临床药理, 2021, 32(8): 1129-1138. doi:10.19378/j.issn.1003-9783.2021.08.010 HUANG Pangning, YANG Huifang, LIU Suli, et al. Mechanisms of Baoyuan Decoction in the treatment of dilated cardiomyopathy based on network pharmacology and molecular docking[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2021, 32(8): 1129-1138. doi:10.19378/j.issn.1003-9783.2021.08.010
[9] 钟晓琴, 宁振求, 王凯, 等.基于网络药理学、分子对接技术与实验验证探讨定志小丸干预阿尔兹海默病的机制研究[J].中药药理与临床, 2023, 39(7):2-9. doi:10.13412/j.cnki.zyyl.20230331.003 ZHONG Xiaoqin, NING Zhenqiu, WANG Kai, et al. Mechanism of Dingzhi Xiaowan against Alzheimer's disease: ased on network pharmacology, molecular docking, and experimental verification[J]. Pharmacology and Clinics of Chinese Materia Medica, 2023, 39(7): 2-9. doi:10.13412/j.cnki.zyyl.20230331.003
[10] 汤柳, 陈君茹, 张玲莉, 等. 基于网络药理学和分子对接技术探讨蛇床子治疗湿疹的作用机制[J]. 现代药物与临床, 2023, 38(3): 540-546. doi:10.7501/j.issn.1674-5515.2023.03.007 TANG Liu, CHEN Junru, ZHANG Lingli, et al. Molecular mechanism of Cnidi Fructus i in treatment of eczema based on network pharmacology and molecular docking[J]. Drugs & Clinic, 2023, 38(3): 540-546. doi:10.7501/j.issn.1674-5515.2023.03.007
[11] 李东棋, 刘慧霞, 田理. 芪丹益肺口服液对变应性鼻炎大鼠肝肾功能指标的影响研究[J]. 亚太传统医药, 2021, 17(3): 66-68. doi:10.11954/ytctyy.202103017 LI Dongqi, LIU Huixia, TIAN Li. Effect of qidan yifei oral liquid on liver and kidney function in allergic rhinitis rats[J]. Asia-Pacific Traditional Medicine, 2021, 17(3): 66-68. doi:10.11954/ytctyy.202103017
[12] 中华中医药学会中药实验药理专业委员会. 变应性鼻炎动物模型制备规范(草案)[J]. 中草药, 2018, 49(1): 50-57. doi:10.7501/j.issn.0253-2670.2018.01.006
[13] 苏荣扎布.蒙医内科学[M]. 北京: 民族出版社, 1986: 487-489
[14] Yang SS, Fu QW, Deng H, et al. Mechanisms and molecular targets of the Yu-Ping-Feng Powder for allergic rhinitis, based on network pharmacology[J]. Medicine, 2021, 100(35): e26929. doi:10.1097/MD.0000000000026929
[15] Noack M, Miossec P. Selected cytokine pathways in rheumatoid arthritis[J]. Semin Immunopathol, 2017, 39(4): 365-383. doi: 10.1007/s00281-017-0619-z
[16] 贾惠静, 薛金梅. Toll样受体激动剂在变应性鼻炎免疫治疗中的研究进展[J]. 中国耳鼻咽喉颅底外科杂志, 2022, 28(5): 107-112. doi:10.11798/j.issn.1007-1520.202221506 JIA Huijing, XUE Jinmei. Development of Toll like receptor agonists for the immunotherapy of allergic rhinitis[J]. Chinese Journal of Otorhinolaryngology-Skull Base Surgery, 2022, 28(5): 107-112. doi:10.11798/j.issn.1007-1520.202221506
[17] 郭剑玲, 李永芳. 依巴斯汀联合孟鲁司特治疗变应性鼻炎的疗效及对炎症因子水平的影响[J]. 航空航天医学杂志, 2022, 33(9): 1091-1093. doi:10.3969/j.issn.2095-1434.2022.09.024 GUO Jianling, LI Yongfang. Effect of ebastine combined with montelukast on allergic rhinitis and its influence on inflammatory factors[J]. Journal of Aerospace Medicine, 2022, 33(9): 1091-1093. doi:10.3969/j.issn.2095-1434.2022.09.024
[18] 黄榆岚, 邓志华, 高思敏, 等. 变应性鼻炎患者血清IL-17、IL-25及TNF-α水平表达及与病情严重程度的关系研究[J]. 现代生物医学进展, 2019, 19(5): 911-914. doi:10.13241/j.cnki.pmb.2019.05.026 HUANG Yulan, DENG Zhihua, GAO Simin, et al. Serum levels of IL-17, IL-25 and TNF-α in Patients with Allergic Rhinitis and their Relationship with Severity of Illness[J]. Progress in Modern Biomedicine, 2019, 19(5): 911-914. doi:10.13241/j.cnki.pmb.2019.05.026
[1] LYU Qian, TANG Yuan, GU Zijun, SHI Sailei, LIU Ping, WAN Wenjin. Current status of benefit finding among patients with allergic rhinitis and its influence factors [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 21-28.
[2] GU Min, LU Meiping. Advances in the application of nanomedicine delivery systems in allergen immunotherapy for allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 106-111.
[3] QIN Nana, LI Yufen, SUN Yuhao, WEI Jian, LI Qin. Effect of interleukin-13 receptor-α2 on nasal mucosal remodeling in rats with allergic rhinitis by TGF-β1/Smad signaling pathway [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 71-77.
[4] DU Kangli, ZHENG Zhenyu, XU Zhanjiang, ZHANG Yu, CHEN Lu, LU Mengyao. Construction and validation of risk prediction model for nasal septal deviation complicated with chronic sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 78-86.
[5] XIONG Qin, ZHANG Yan, WU Rina, LI Feng, TANG Lixing. Application of intranasal corticosteroids in pediatrics [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 160-167.
[6] XU Xuemeng, FAN Lei, YU Wangbo, JIANG Zhiyue, PAN Chen, HUANG Yongqin. Meta-analysis of the efficacy of omalizumab in combination with specific immunotherapy for allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 26-33.
[7] HUANG Huan, HUA Hongli, DENG Yuqin, JIANG Chengyang, WANG Yuwei, YANG Xinghai. Correlation of allergic rhinitis, tonsil adenoid hypertrophy, and sinusitis in children and analysis of its clinical guiding value [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 34-41.
[8] LIU Yijun, GU Yue, GUAN Dayu, YANG Yucheng, SHEN Yang. The long-term clinical efficacy and safety of vidian neurectomy in refractory allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 42-48.
[9] ZHANG Ting, WANG Meilan, GAO Yingqin. Research progress of IL-35 in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 139-147.
[10] ZHANG Zhuping, PENG Zican, XIAO Zhenlong, LI Cheng, YU Di, WANG Xinglong, CHEN Wei, Guo Bei. The value of a novel nasal secretion eosinophil cationic protein-myeloperoxidase test paper assay in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(3): 129-134.
[11] WANG Xin, YUAN Jingjing, HAO Pengpeng, YAN Zhanfeng, LIU Jianhua, ZHONG Liqun, LIU Siming, WU Wei, ZHAO Xiaoyang, ZHANG Ying. Protective effect of Chinese herbal medicine on sudden sensorineural hearing loss: a retrospective analysis and network pharmacology [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 6-17.
[12] LIU Chang, YANG Jingpu, GAO Yu, WANG Wenjia. Analysis of allergen detection results in children with allergic rhinitis in the Changchun area [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 51-58.
[13] ZHOU Heqing, SHEN Qi. Research progress on biomarkers of traditional Chinese medicine in the treatment of allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 168-176.
[14] WANG Manxian, ZHENG Quan, YANG Liang. Research progress in the treatment of allergic rhinitis with bacterial lysates [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 141-145.
[15] LIU Chang, FANG Hongyan, LIU Xiao, FU Dongna, WANG He, WANG Jing, YANG Jingpu. Analysis of sensitization characteristics of Artemisia pollen in autumn allergic rhinitis in the Changchun area of China [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 13-19.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd