山东大学耳鼻喉眼学报

• 论文 • 上一篇    下一篇

Skp2与P27kip1在鼻咽癌组织中的表达及意义

王 丽1,路永辉2   

  1. 1.青岛大学医学院附属烟台毓璜顶医院耳鼻咽喉-头颈外科, 山东 烟台264000;2. 滨州医学院教务处, 山东 滨州 256603
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-04-24 发布日期:2007-04-24
  • 通讯作者: 王 丽

Expression and significance of Skp2 and P27kip1 protein in nasopharyngeal carcinoma

WANG Li1, LU Yong-hui2   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Yantai Yuanghuangding Hospital,Medical College of Qingdao University, Yantai 264000, Shandong, China; 2. Academic Affairs Office,Binzhou Medical College, Binzhou 256603, Shandong, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24
  • Contact: WANG Li

摘要: 目的:F-box蛋白家族中的一员Skp2参与细胞周期抑制蛋白P27kip1泛素化降解,研究发现Skp2在乳腺癌、胃癌、前列腺癌等多种恶性肿瘤中表达增加。本研究旨在探讨Skp2与P27kip1在鼻咽癌组织中的表达及与鼻咽癌多项临床病理特征的关系,并探讨两者的相关性。方法:鼻咽癌组39例,慢性鼻咽炎组24例。标本均经石蜡包埋,HE染色确诊。Skp2和P27kip1蛋白的检测采用免疫组化染色(PV-9000二步法)。结果:(1) Skp2在鼻咽癌组织中的阳性表达率为71.79%,明显高于其在慢性鼻咽炎组织中的阳性表达率(41.67%),差异有统计学意义(P<0.05);P27kip1在鼻咽癌组织中的阳性表达率为35.90%,明显低于其在慢性鼻咽炎组织中的阳性表达率(79.17%),差异有统计学意义(P<0.01);(2) Skp2、P27kip1的蛋白表达与鼻咽癌的临床分期、颅神经侵犯有关(P<0.05),与年龄、性别、分化程度、颈淋巴结转移无关(P>0.05);(3) Skp2、P27kip1在鼻咽癌组织中的表达呈负相关(rs=-0.373,P=0.019)。结论:鼻咽癌中Skp2蛋白表达与靶蛋白P27kip1蛋白降解有关,Skp2、P27kip1可能与鼻咽癌的发生发展有关,联合检测Skp2、P27kip1蛋白表达可作为判断鼻咽癌生物学行为的指标之一。

关键词: 鼻咽肿瘤, Skp2, 蛋白, P27kip1, 蛋白, 细胞周期

Abstract: Objective: The F-box protein Skp2 is required for the ubiquitin-mediated proteolysis of the cyclin-dependent kinase inhibitor P27kip1. Overexpression of Skp2 has been reported in many cancers including breast carcinoma, gastric carcinoma and prostate cancer. The purpose of this study is to investigate the correlation of Skp2 and P27kip1 expression with the clinical pathological features of nasopharyngeal carcinoma, and the correlation between expression of Skp2 and P27kip1 in nasopharyngeal carcinoma. Methods: Thirty-nine cases of nasopharyngeal carcinoma and twenty-four cases of chronic nasopharyngitis were enrolled in this experiment. All specimens were proved by formallin-fixed paraffin-embedded slides with the hematoxylin eosin staining. Expression of Skp2 and P27kip1 was determined by using an immunohistochemical staining (PV-9000 two-step kit). Result: The Skp2 labeling frequency (71.79%) in nasopharyngeal carcinoma was significantly higher than that (41.67%) in chronic nasopharyngitis(P<0.05). The positive rate of P27kip1 expression in nasopharyngeal carcinoma was 35.90%, which was remarkably lower than that (79.17%) in chronic nasopharyngitis (P<0.01). Expression of Skp2 and P27kip1 protein in nasopharyngeal carcinoma was correlated with the clinical stage and cranial nerve encroachment (P<0.05), but not correlated with the patient age, sex, histological grade, or lymph node metastases (P>0.05). Skp2 expression was inversely correlated with P27kip1 in nasopharyngeal carcinoma(rs=-0.373, P=0.019). Conclusion: Expression of Skp2 protein may decrease the P27kip1 level in human nasopharyngeal carcinoma, indicating it is involved in the development of human nasopharyngeal carcinoma. Skp2 and P27kip1 may be used as hopeful markers for predicting the clinical pathological features and the prognosis of these patients.

Key words: Nasopharyngeal carcinoma, Skp2, protein, P27kip1, protein, Cell cycle

中图分类号: 

  • R739.65
[1] 张炜,曾昱菡,余先崧. 慢性鼻窦炎手术前后ECP、EGF、IL-6的水平变化及临床意义[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 63-67.
[2] 张会芳,杨红珍. 经鼻持续气道正压通气对阻塞性睡眠呼吸暂停低通气[J]. 山东大学耳鼻喉眼学报, 2018, 32(2): 48-51.
[3] 任涛,唐以军,王梅芳,刘岩,钱鑫. 阻塞性睡眠呼吸暂停低通气综合征对血糖、血脂及C反应蛋白的影响[J]. 山东大学耳鼻喉眼学报, 2017, 31(6): 53-57.
[4] 耿聪,张丽伟,丁明. 全身麻醉对单纯鼾症患者术后认知功能的影响[J]. 山东大学耳鼻喉眼学报, 2017, 31(5): 85-88.
[5] 杜志宏. 电子鼻咽镜下口鼻联合径路在鼻咽部活检中的应用[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 117-118.
[6] 程雷,钱俊俊,田慧琴. 变应性鼻炎研究的若干进展[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 1-3.
[7] 吴中飞,怀德,桂晓钟,赵新,周文成,袁源,田慧琴,陆美萍,程雷. 苏皖健康人群血清免疫球蛋白E水平测定与分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 50-54.
[8] 张彦惠综述, 董频审校. Argonaute2蛋白及其在人类肿瘤研究中的新进展[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 95-99.
[9] 周荧,徐亚如,黄骁辰,陈渊,章明炯,张大为,唐奇,朱进,陈仁杰. 靶向c-Met嵌合抗原受体T细胞的制备及其对鼻咽癌细胞作用的研究[J]. 山东大学耳鼻喉眼学报, 2017, 31(2): 49-54.
[10] 邓月,徐亚平,彭浒,陶绮蕾,范静平,孙爱华,吴建. 铁必复颗粒治疗缺铁感音神经性聋患者的疗效分析[J]. 山东大学耳鼻喉眼学报, 2017, 31(1): 75-79.
[11] 葛晓辉,王宁宇,占小俊,王春燕,王建亭,王辉,郝荣颖,马坤,刘萍,李丽. 手术对阻塞性睡眠呼吸暂停低通气综合征患者血脂代谢变化的临床观察[J]. 山东大学耳鼻喉眼学报, 2017, 31(1): 27-30.
[12] 刘叶,孙殿水,殷进军,夏明,徐英,孙建华. 鼻咽癌调强放疗后前庭功能损伤的机制研究[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 14-17.
[13] 高天喜,夏翠,闫静,侯瑾,康全清,王正辉,任晓勇. 间歇低氧模型大鼠肝脏组织中IGF-1、IGFBP-3的表达及分析[J]. 山东大学耳鼻喉眼学报, 2016, 30(5): 38-41.
[14] 余鹏举, 许风雷, 杨军, 陈伟, 江满杰. 血浆1-3-β-D葡聚糖对真菌性鼻窦炎的诊断价值[J]. 山东大学耳鼻喉眼学报, 2016, 30(4): 94-97.
[15] 周文成,袁源,程雷. 尘螨变应性鼻炎特异性免疫治疗的疗效预测指标探讨[J]. 山东大学耳鼻喉眼学报, 2016, 30(4): 43-49.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!