下咽癌中差异表达的蛋白激酶及其抑制剂的生物信息学筛选

doi:10.6040/j.issn.1673-3770.0.2015.340

Abstract

Abstract: Objective To screen differentially expressed protein kinases and their inhibitors, in order to provide new targets for molecular therapy of hypopharyngeal cancer. Methods GEO database and SAM software were employed to screen the differentially expressed protein kinase genes in hypopharyngeal cancer. Human hypopharyngeal cancer FaDu cell line was cultured in vitro. Real-time PCR was used to prove the accuracy of microarray results. Based on KEGG database, the kinase-regulated pathways were identified. The inhibitors of such kinases were identified by using kinase-inhibitor database. Results A total of 3 protein kinase genes(PKC-β, CDK6 and CDC42)were identified(fold change≥2.0, P<0.05). Real-time PCR showed that the 3 kinase genes were differentially expressed in FaDu cells, which was consistent with the microarray results(P<0.05)Pathway analysis indicated that a complex pathway network was regulated by the 3 kinases. The results of inhibitor screening showed that 5 inhibitors regulated PKC-β, 4 inhibitors regulated CDK6 and 3 inhibitors regulated CDC42. There were less than 10 studies about the 4 inhibitors in cancer. Conclusion Three differentially expressed protein kinases(PKC-β, CDK6 and CDC42)are identified and they are able to promote the development of tumor. Their kinase inhibitors may play a potential anti-cancer role, which may provide a new target for molecular therapy of hypopharyngeal cancer.

Key words: Bioinformatics, Protein kinase, Kinase inhibitor, Hypopharyngeal cancer

CLC Number:

R739.65

HU Wenliang, ZHENG Yanqiu, CUI Xiaobo, CUI Yanru, SUN Yuanhao. Bioinformatic screening of differentially expressed protein kinases and their inhibitors in hypopharyngeal cancer.[J].JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(3): 24-28.

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Bioinformatic screening of differentially expressed protein kinases and their inhibitors in hypopharyngeal cancer.

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