JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (5): 41-44.doi: 10.6040/j.issn.1673-3770.0.2017.204

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Long-term low intensity noise exposure reduce the number of ribbon synapses in guinea pigs.

SONG Feng1, GAN Bin1, XU Anting2, WANG Jian3   

  1. 1. School of Clinical Medicine, Shandong University, Jinan 250012, Shandong, China;2. Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China;3. Institute of Hearing, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China
  • Received:2017-05-11 Online:2017-10-16 Published:2017-10-16

Abstract: Objective To observe the possible loss and recovery of ribbon synapses in the cochlea of guinea pigs repeatedly exposed to low-intensity noise. Methods Guinea pigs were exposed to 95 dB sound pressure level of white noise for 4 hours per day, continuously for 7 days. The animals were then divided into three groups, and respectively named group 1DPN(1 day post-noise), 1WPN(1 week post-noise), and 1MPN(1 month post-noise), with each group containing 10 guinea pigs. Audiological examinations(auditory brainstem response)and immunofluorescence staining were performed,and the number of ribbon synapses were counted by observing double staining results of C-terminal-binding protein 2 and postsynaptic density protein 95 labeling. Results After long-term low-intensity noise exposure, the auditory brainstem responsethreshold of group 1DPN significantly increased(P<0.05)compared to the control group. No apparent changes in hair cell shape, arrangement, or number were observed, but the number of ribbon synapses was statistically significant(P<0.05). With the extension of time after leaving the noisy environment, the ABR threshold and number of synapses recovered gradually. Conclusion Repeated exposure to long-term, low-intensity noise can cause hearing loss, which is reversible after leaving the noisy environment. Reduction in the number of inner hair cell ribbon synapses caused by long-term low-intensity noise exposure can restore partially while in a noise-free environment.

Key words: Ribbon synapse, Auditory brainstem response, Low intensity noise

CLC Number: 

  • R764.43
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