Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (2): 26-31.doi: 10.6040/j.issn.1673-3770.0.2021.090

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Transcriptomic analysis of glutamine deprivation on laryngeal carcinoma cells

WANG Xiaoting, CHEN Zhengnong, YI Hongliang   

  1. Department of Otorhinolaryngology & Head and Heck Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital / Shanghai Key Laboratory of Sleep Disordered Breathing / Otolaryngology Institute of Shanghai Jiao Tong University, Shanghai 200233, China
  • Published:2022-04-15

Abstract: Objective Glutamine is necessary for head and neck cancer cells to maintain maximum proliferation rate, this article used RNA sequence to explore the effect of glutamine deprivation on laryngeal cancer cell gene expression Changes. Methods The CCK8 assay measured laryngeal cancer cell proliferation rate, RNA sequence measured transcriptome changes of laryngeal cancer cells after glutamine deprivation, bioinformatics methods analyzed gene enrichment. Results Glutamine deprivation restricted laryngeal cancer cell proliferation, transcriptome analysis found that glutamine deprivation leads to 328 gene up-regulated and 210 genes down-regulated. GO analysis showed that the differentially expressed genes were mainly related to cell metabolism, organelle synthesis, protein and nucleotide synthesis. KEGG signaling pathway analysis showed that signaling pathways changes were mainly related to O-polysaccharides and glycosylphosphatidylinositol biosynthesis, fructose mannacil amino acids and nucleotides metabolism, and the P53 signaling pathway. Disease enrichment found that the differentially expressed genes were mainly related to head and neck carcinoma. Conclusion Glutamine deprivation restricts nucleic acid metabolism, RNA and protein binding, and affects the P53 signaling pathway.

Key words: Laryngeal cancer, Glutamine metabolism, RNA sequence

CLC Number: 

  • R767.91
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[4] To investigate the swallowing function recovery on 23 cases of supraglottic laryngeal carcinoma postoperation. MethodsFrom 2016 to 2019, a retrospective swallowing function analysis was performed on 23 cases of supraglottic laryngeal carcinoma. All of the patients underwent horizontal hemilaryngectomy and reconstruction with tongue flap. The M.D. Anderson Dysphasia Inventory(MDADI)was used to evaluate the swallowing function from emotional dimension, social function dimension, and physiological dimension before and after operation. Results2 patients died during follow-up period.1 case lost follow-up. 20 patients recovered to normal diet. The overall swallowing median score was 90.00[80.00,100.00], and the postoperative score median was 80.00[80.00,80.00](P=0.359).The median score of emotion dimension was 91.67[77.50,93.33], 80.00[80.00,83.33](P=0.065)after operation. The median score of social function was 90.00[80.00,96.00]before surgery, and 84.00[80.00,92.00](P=0.587)after surgery. Physiological function median score was 83.75[76.25,89.38], 80.00[75.00,84.38](P=0.018)after surgery. ConclusionTongue flap can improve the swallowing function and the quality of patient life after the horizontal hemilaryngectomy.. The swallowing function recovery study on tongue flap after horizontal hemilaryngectomy [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(5): 127-131.
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