Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (6): 71-77.doi: 10.6040/j.issn.1673-3770.0.2024.152

• Original Article • Previous Articles    

Effect of interleukin-13 receptor-α2 on nasal mucosal remodeling in rats with allergic rhinitis by TGF-β1/Smad signaling pathway

QIN Nana1, LI Yufen2, SUN Yuhao1, WEI Jian3, LI Qin2   

  1. 1. School of Clinical Medicine, Shandong Second Medical University, Weifang 261000, Shandong, China2. Department of Otorhinolaryngology & Head and Neck Surgery, Linyi People's Hospital, Linyi 276000, Shandong, China3. Department of Otolaryngology, Linyi Hospital of Traditional Chinese Medicine, Linyi 276000, Shandong, China
  • Published:2025-11-19

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Abstract: Objective To investigate the effect of interleukin-13 receptor α2(sIL-13Rα2)on nasal mucosal remodeling in rats with allergic rhinitis(AR)and its possible mechanism. Methods Rats were divided into AR model group, sIL-13Rα2 treatment group and control group by random number table method, 10 rats in each group. After constructing the Wistar rat AR model with ovalbumin and aluminum hydroxide in the AR model group and sIL-13Rα2-treated group of rats, 50 μL of phosphate buffer and 50 μL of sIL-13Rα2(100 μg)were dripped into the nasal cavity on each side of each rat from the 4 th to the 12th week, respectively, twice a week.Nasal mucosa tissues of rats were taken 24 h after the end of the last drop, and were stained with hematoxylin-eosin staining to observe the pathological changes. Reverse Transcription-Polymerase Chain Reaction was used to detect the levels of TGF-β1, Smad2, Smad3 and Smad7 mRNA in the nasal mucosa tissues of rats in each group, and Western blot was used to detect the expression of TGF-β1, Smad2, Smad3 and Smad7 proteins. Results The nasal mucosa of rats in AR model group was swelled, basement membrane thickened, epithelial cells arranged disorderly, interstitial edema, and infiltration of a large number of inflammatory cells, whereas the above inflammatory changes were significantly reduced in sIL-13Rα2 group rats. The intensity of TGF-β1, Smad2 and Smad3 mRNA expression in the nasal mucosa of sIL-13Rα2 group rats was significantly lower than that of AR model group rats(P<0.001, P<0.001, P<0.001).The intensity of Smad7mRNA expression in the nasal mucosa tissue of sIL-13Rα2 group was significantly higher than that of AR model group(P<0.001). The protein expression of TGF-β1, Smad2, and Smad3 in the nasal mucosal tissues of rats in the sIL-13Rα2 treatment group was significantly lower than that in the AR model group(P<0.05, P<0.001, P<0.001). The protein expression of Smad7 in the nasal mucosal tissues of rats in the sIL-13Rα2 treatment group was significantly higher than that in the AR model group(P<0.01). Conclusion Instillation of IL-13Rα2 into nasal cavity can significantly reduce the remodeling of nasal mucosa in AR rats by inhibiting the overexpression of TGF-β1, Smad2 and Smad3 and up-regulating the expression of Smad7 in nasal mucosa.

Key words: Model animals, Allergic rhinitis, Interleukin 13 receptor α2, Transforming growth factor-β1, Smad, Remodeling

CLC Number: 

  • R765
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