Der p2 B细胞表位RNA疫苗制备及其对过敏性鼻炎小鼠特异性IgE抑制作用

doi:10.6040/j.issn.1673-3770.0.2024.170

Abstract

Abstract: Objective This study aimed to construct a house dust mite allergen Der p 2 B cell epitope RNA vaccine and evaluate its inhibitory potency against antigen-specific IgE in allergic rhinitis mice. Methods Epitope prediction software was utilized to predict potential B-cell epitope peptides on the Der p2 protein sequence, followed by synthesis of these peptides and analysis of their IgE reactivity in vitro. The B-cell epitope peptide was then linked with a carrier protein to construct the B-cell epitope RNA vaccine, which was administered to allergic rhinitis mice via subcutaneous injection. Levels of specific IgE, IgG4, Treg, and Th17 cells in the peripheral blood of the mice were subsequently tested. Results Compared with the recombinant Der p2 positive control group, the predicted four B cell epitope peptides exhibited significantly lower IgE reactivity(P<0.001). Furthermore, treatment with the Der p2 B cell epitope RNA vaccine led to a significant decrease in serum levels of specific IgE and an increase in IgG4 compared to mice in the allergic rhinitis group(P<0.05). Additionally, there was an increase in Treg ratio and a reduction in Th17 ratio after treatment(P<0.05). Conclusion The construction of the Der p2 B cell epitope RNA vaccine has been successfully achieved and it effectively reduces specific IgE levels while increasing neutralizing antibody levels and Treg ratios in peripheral blood of allergic rhinitis mice.

Key words: house dust mite, B cell epitope, RNA vaccine, specific IgE

CLC Number:

R765.21

YANG Gui, HU Tianyong, HE Haipeng, MA Li, WEI Haifeng, HUANG Jiamin, LIU Zhiqiang. Preparation of a Der p2 B cell epitope RNA vaccine and its specific IgE inhibition in mice with allergic rhinitis[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 6-12.

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Preparation of a Der p2 B cell epitope RNA vaccine and its specific IgE inhibition in mice with allergic rhinitis

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