不同发育时期小鼠初级听觉皮层中芳香化酶及雌激素受体表达与听力的相关性

doi:10.6040/j.issn.1673-3770.0.2024.517

Abstract

Abstract: Objective To investigate the expression and potential regulatory roles of aromatase(AROM)and three estrogen receptors(ERs)—estrogen receptor alpha(ERα), estrogen receptor beta(ERβ), and G protein-coupled receptor 30(GPR30)—in the primary auditory cortex(A1)during auditory development. Methods Male C57BL/6J mice at different postnatal stages—postnatal day 7(P7), P14, P21, P30, and P60-were selected. Auditory thresholds were measured by auditory brainstem response(ABR), and qRT-PCR, Western bloting, and immunohistochemistry were performed to examine the expression changes of AROM and ERs in the A1. Spearman correlation analysis was conducted between expression levels and hearing thresholds. Results ABR thresholds decreased significantly from P14 onward(P<0.05), reaching maturity at P30. qPCR revealed that the expression of AROM, GPR30, and ERβ increased with age(P<0.05)while ERα peaked at P30(P<0.05). Protein levels(Western bloting and immunohistochemistry)confirmed a significant developmental increase in AROM and ERs expression(P<0.05). Spearman correlation analysis showed strong correlations between the developmental changes in hearing thresholds and the expression of AROM(∣r_s∣=0.75)and ERβ(∣r_s∣=0.72), a weak correlation with GPR30(∣r_s∣=0.64), and no significant correlation with ERα. Conclusion Postnatal auditory development in mice progressively matures, accompanied by increasing expression levels of AROM, GPR30, and ERβ in the A1. The AROM-GPR30/ERβ axis is suggested to potentially facilitate the establishment and maturation of auditory function in mice.

Key words: Aromatase, 17β-Estradiol, Estrogen receptors, ERα, ERβ, G protein coupled receptor 30, Auditory cortex

CLC Number:

R339.16

TANG Xi, HUANG Maoling, ZHANG Jiqiang, ZHONG Cheng. Correlation between aromatase and estrogen receptor expression in the primary auditory cortex of mice at different developmental stages and hearing ability[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 46-53.

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Correlation between aromatase and estrogen receptor expression in the primary auditory cortex of mice at different developmental stages and hearing ability

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