Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (5): 1-7.doi: 10.6040/j.issn.1673-3770.0.2023.382

• Original Article •    

Electrophysiological characteristics of deep projections from bilateral auditory cortex

LU Jiantao, ZHONG Jie, LIU Shaofeng   

  1. Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou 511300, Guangdong, China
  • Published:2025-09-19

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Abstract: Objective This study aimed to investigate the electrophysiological characteristics of deep projections from bilateral auditory cortices, with a focus on the excitatory pyramidal neurons and their projections to the contralateral auditory cortex via the corpus callosum. The goal was to gain a better understanding of the projection localization of auditory information in bilateral auditory brain regions and the electrophysiological properties of target neurons. Methods Mice were used as the experimental subjects. Optogenetic viruses were injected into the auditory cortex to manipulate the activity of the corpus callosum. Electrophysiological and immunohistochemistry techniques were employed. Results The primary regions where deep neurons in the auditory cortex projected via the corpus callosum were layer 5B and layer 6 of the contralateral auditory cortex. Both layers exhibited monosynaptic connections, although the extent of information input, electrophysiological properties, and morphology differed significantly. Conclusion Direct fiber connections exist between deep neurons in bilateral auditory cortices. The projections to layer 5B and layer 6 of the contralateral side represent two distinct types of excitatory neurons, which likely contribute to different regulatory roles in auditory information processing.

Key words: Auditory cortex, Corpus callosum, Pyramidal neurons, Electrophysiology, Optogenetics

CLC Number: 

  • R764.4
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